| 长江中下游黄鳝遗传多样性的微卫星分析 |
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| 引用本文： | 周宇芳,胡杭娇,张龙韬,舒妙安.长江中下游黄鳝遗传多样性的微卫星分析[J].生物技术通报,2011(11). |
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| 作者姓名： | 周宇芳 胡杭娇 张龙韬 舒妙安 |
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| 作者单位： | 浙江大学动物科学学院,杭州,310058 |
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| 基金项目： | 国家公益性行业农业专项(201003076) |
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| 摘 要： | 为了解我国长江中下游地区野生黄鳝(Monopterus albus)的种质资源现状,利用黄鳝微卫星分子标记分析我国长江中下游4个黄鳝野生群体(湖北群体、安徽群体、江苏群体和浙江群体)的遗传多样性水平.通过磁珠富集法获得50个黄鳝徽卫星序列,设计并合成了30对微卫星引物,经筛选得到8对多态性稳定的引物,均为高度多态位点.每对引物扩增得到等位基因数12 -26,平均等位基因数17.4个群体的平均多态信息含量分别为0.804、0.864、0.824和0.736,平均等位基因数分别为9.13、11.00、9.00和7.25,平均期望杂合度分别为0.865、0.918、0.882和0.813,表明4个黄鳝群体遗传多样性丰富,其中安徽群体遗传多样性水平最高,浙江群体相对较低.4个群体间遗传分化指数(FsT)为0.031 2-0.096 5,6.28％的遗传变异存在于群体间,表明4个群体间存在一定的遗传分化.聚类分析显示,浙江群体与安徽群体先聚在一起,再与江苏群体聚为一支,湖北群体单独聚为一支.
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| 关 键 词： | 黄鳝 磁珠富集法 微卫星标记 遗传多样性 |
Microsatellite Analysis of Genetic Diversity of Monopterus albus Along the Middle and Lower Reaches of Yangtze River Basin |
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| Zhuo Yufang,Hu Hangjiao,Zhang Longtao,Shu Miaoan.Microsatellite Analysis of Genetic Diversity of Monopterus albus Along the Middle and Lower Reaches of Yangtze River Basin[J].Biotechnology Bulletin,2011(11). |
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| Authors: | Zhuo Yufang Hu Hangjiao Zhang Longtao Shu Miaoan |
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| Institution: | Zhuo Yufang Hu Hangjiao Zhang Longtao Shu Miaoan(College of Animal Science,Zhejiang University,Hangzhou 310058) |
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| Abstract: | In order to explore the genetic diversity and genetic structure of Monopterus albus in different areas,we had developed some microsatellite markers with magnetic beads,and used them to evaluate genetic variation in four wild populations of Monopterus albus(Hubei,Anhui,Jiangsu and Zhejiang)along the middle and lower reaches of the Yangtze River Basin.Sixty-four positive clones were selected and sequenced,getting fifty microsatellite sequences.Thirty pairs of primers were synthesized,and eight polymorphic mic... |
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| Keywords: | Monopterus albus Enrichment by magnetic beads Microsatellite markers Genetic diversity |
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