| CUP1为筛选标记的酿酒酵母整合型多基因表达载体的构建 |
| |
| 引用本文： | 阳辛凤,徐林,弓淑芳,郭安平,孔华,贺立卡.CUP1为筛选标记的酿酒酵母整合型多基因表达载体的构建[J].生物技术通报,2011(10). |
| |
| 作者姓名： | 阳辛凤 徐林 弓淑芳 郭安平 孔华 贺立卡 |
| |
| 作者单位： | 1. 海南大学农学院,海口570228;中国热带农业科学院热带生物技术研究所,海口571101;中国热带农业科学院分析测试中心,海口571101
2. 中国热带农业科学院热带生物技术研究所,海口,571101 |
| |
| 基金项目： | 国家“863”重点课题(2007AA021307); 转基因生物新品种培育重大专项课题(2008ZX08012-04) |
| |
| 摘 要： | 为了赋予工业酿酒酵母对淀粉和纤维素的降解活性,提高酿酒酵母对粗木薯粉进行酒精发酵时的酒精产率；另一方面,为了解决工业酿酒酵母不适于使用营养缺陷型筛选标记对转化子进行筛选的问题,以及避免引入抗药性标记基因带来的安全性问题,构建了以抗铜蛋白基因CUP1为筛选标记的酿酒酵母整合型多基因表达载体.以载体pYES2-PMF-rDNA为基础,以新的筛选标记基因CUP1替换原有的尿嘧啶Ura-基因,得到载体pYES2M.再顺序插入葡聚糖内切酶基因eg3、葡萄糖淀糖酶基因gal和β-葡萄糖苷酶基因bgl1,构建得到以CUP1为筛选标记的酵母整合型三价表达载体pYES2M-eg3-ga1 -bgl1,其中每个基因都具有独立而完整的表达盒,包括启动子、信号肽和终止子,从而实现多基因单表达载体一次转化.
|
| 关 键 词： | 酿酒酵母 载体 CUP1 多基因转化 |
Construction of an Integrated Muiti-gene Expression Vector of Saccharomyces cerevisiae Using CUP1 as Selection Marker |
| |
| Yang Xinfeng,Xu Lin,Gong Shufang,Guo Anping,Kong Hua,He Lika.Construction of an Integrated Muiti-gene Expression Vector of Saccharomyces cerevisiae Using CUP1 as Selection Marker[J].Biotechnology Bulletin,2011(10). |
| |
| Authors: | Yang Xinfeng Xu Lin Gong Shufang Guo Anping Kong Hua He Lika |
| |
| Institution: | Yang Xinfeng1,2,3 Xu Lin2 Gong Shufang2 Guo Anping2 Kong Hua2 He Lika2(1Agricultural College,Hainan University,Haikou 570228,2Institute of Tropical Bioscience and Biotechnology CATAS,Haikou 571101,3Analysis and Test Center of CATAS,Haikou 571101) |
| |
| Abstract: | In order to make industrial Saccharomyces cerevisiae possessing degradation function to starch and cellulose,improve alcohol yield as alcohol fermentation on crude cassava by Saccharomyces cerevisiae,resolve the problem of auxotrophic selection marker not fitting for the screening of industrial S.cerevisiae transformants and biosafety of drug-resistant marker genes,a yeast integrated multi-gene expression vector was constructed using the copper resistance gene CUP1 as selection marker.Based on the vector pY... |
| |
| Keywords: | Saccharomyces cerevisiae Vector CUP1 gene Multi-gene transformation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
