| M yb28基因表达载体的构建及拟南芥转化 |
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| 引用本文： | 任莉,刘慧英,王华森,朱祝军.M yb28基因表达载体的构建及拟南芥转化[J].生物技术通报,2012,0(3):80-84. |
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| 作者姓名： | 任莉 刘慧英 王华森 朱祝军 |
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| 作者单位： | 1. 石河子大学园艺系,石河子,832000
2. 浙江农林大学农业与食品科学学院,杭州,311300 |
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| 基金项目： | 国家自然科学基金项目,浙江省基金项目,浙江农林大学启动基金项目 |
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| 摘 要： | 利用通过RT-PCR扩增到的M yb28(GenBank注册号:HQ270468)基因分别构建正义和反义植物表达载体,采用冻融法转入农杆菌LBA4404菌株,通过花序浸泡法对Myb28基因缺失的拟南芥进行了遗传转化,经RT-PCR和酶切鉴定,结果表明Myb28正义和反义真核表达载体构建成功,经基因组PCR鉴定表明正义表达载体已成功整合到拟南芥基因组中。
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| 关 键 词： | 小白菜 M yb28 硫代葡萄糖苷 表达载体 拟南芥 |
Construction of Expression Vectors of Brassica chinensis Myb28 Gene and Transformation to Arabidopsis thaliana |
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| Ren Li
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Liu Huiying
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Wang Huasen
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Zhu Zhujun.Construction of Expression Vectors of Brassica chinensis Myb28 Gene and Transformation to Arabidopsis thaliana[J].Biotechnology Bulletin,2012,0(3):80-84. |
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| Authors: | Ren Li Liu Huiying Wang Huasen Zhu Zhujun |
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| Institution: | 1Department of Horticulture,Shihezi University,Shihezi 832000;2Department of Horticulture,School of Agriculture and Food Science,Zhejiang Agriculture & Forestry University,Hangzhou 311300) |
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| Abstract: | A full-length cDNA encoding Myb28 from Brassica chinensis was isolated using RT-PCR.The recombinant plasmids were transferred into Agrobacterium tunefacien LBA4404 by freeze-thaw method,and then were used to infect mutant Arabidopsis thaliana.Using PCR,we proved that the sense and antisense expression vector were constructed successfully and the genes were integrated into Arabidopsis thaliana. |
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| Keywords: | Brassica campestris ssp chinensis var communis Myb28 Glucosinolates Expression vector Arabidopsis thaliana |
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