| Providencia rettgeri strain HNR菌株羟胺氧化酶超声波法提取的研究 |
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| 引用本文： | 黄珏,何义亮,赵彬,Shauna Taylor,张文英.Providencia rettgeri strain HNR菌株羟胺氧化酶超声波法提取的研究[J].生物技术通报,2008(Z1). |
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| 作者姓名： | 黄珏 何义亮 赵彬 Shauna Taylor 张文英 |
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| 作者单位： | 上海交通大学环境科学与工程学院,上海,200240 |
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| 基金项目： | 国家自然科学基金项目(No.20676078) |
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| 摘 要： | 为了更有效和便捷的提取菌株脱氮过程中的关键酶,本文利用超声波破碎法对异养脱氮菌Providencia rettgeri strain HNR进行破碎,通过单因素实验及正交试验,确定出最佳破碎条件,便于后续试验。实验结果表明:最佳超声破碎条件为菌液OD_(600)为1.996,工作次数为70次,工作/间歇为5/5s功率为400W的组合为最佳工作条件。在此工作条件下破碎后得到的酶粗提液对羟胺有降解,得到羟胺氧化酶。
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| 关 键 词： | 超声波法 细胞破碎 Providencia rettgeri strain HNR |
Study on Hydroxylamine Oxidase Extraction of Providencia rettgeri strain HNR with Ultrasonic Cell-break Method |
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| Huang Jue,He Yiliang,Zhao Bin,Shauna Taylor,Zhang Wenying.Study on Hydroxylamine Oxidase Extraction of Providencia rettgeri strain HNR with Ultrasonic Cell-break Method[J].Biotechnology Bulletin,2008(Z1). |
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| Authors: | Huang Jue He Yiliang Zhao Bin Shauna Taylor Zhang Wenying |
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| Institution: | School of Environmental Science and Engineering;Shanghai Jiao Tong University;Shanghai 200240 |
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| Abstract: | In order to obtain the key enzyme involved in the nitrogen removal process of Providencia rettgeri strain HNR effectively and conveniently,the ultrasonic cell-disruption technique is used.Optimum conditions were chosen based on single-factor and orthogonal experimental results.The results showed that optimum conditions for cell-breakage of Providencia rettgeri strain HNR via ultrasonication were:cell OD_(600)1.996,work times 70,work/interval time 5s/5s and ultrasonic output power 400W.The hydroxylamine oxid... |
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| Keywords: | Providencia rettgeri strain HNR |
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