| 转基因水稻“科丰6号”实时荧光PCR定性定量检测方法研究 |
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| 引用本文： | 黄新,张琰,侯立华,张祺,陈洪俊,朱水芳.转基因水稻“科丰6号”实时荧光PCR定性定量检测方法研究[J].生物技术通报,2010(2). |
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| 作者姓名： | 黄新 张琰 侯立华 张祺 陈洪俊 朱水芳 |
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| 作者单位： | 中国检验检疫科学研究院动植物检疫研究所,北京,100029 |
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| 基金项目： | 转基因生物新品种培育重大专项课题，国家"863"计划 |
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| 摘 要： | 旨在建立转基因水稻"科丰6号"外源基因和边界序列的实时荧光PCR检测方法,为科丰6号定性定量检测提供技术支持。根据外源基因和边界序列信息,设计实时荧光PCR探针引物,优化体系,对不同转基因产品和不同转基因含量的"科丰6号"水稻进行检测。结果显示,所设计的引物探针具有很好的特异性,与其他转基因水稻品系、转基因玉米、转基因棉花、转基因番茄和非转基因水稻均无非特异性反应,对转基因水稻"科丰6号"的检测灵敏度达到0.01%。建立的科丰6号实时荧光PCR检测方法重复性好、灵敏度高,能够达到目前国际上转基因产品定量检测的标准,为该水稻品系的定性定量检测提供技术支持。
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| 关 键 词： | "科丰6号" 实时荧光PCR 定量检测 |
The Quantitative Real-time PCR Detection of Genetically Modified Rice kefeng No.6 |
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| Huang Xin,Zhang Yan,Hou Lihua,Zhang Qi,Chen Hongjun,Zhu Shuifang.The Quantitative Real-time PCR Detection of Genetically Modified Rice kefeng No.6[J].Biotechnology Bulletin,2010(2). |
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| Authors: | Huang Xin Zhang Yan Hou Lihua Zhang Qi Chen Hongjun Zhu Shuifang |
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| Institution: | Huang Xin Zhang Yan Hou Lihua Zhang Qi Chen Hongjun Zhu Shuifang (Institute of Animal , Plant Quarantine,Chinese Academy of Inspection , Quarantine,Beijing 100029) |
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| Abstract: | It was to establish the quantitative detection method for genetically modified ricekefeng No.6by real-time PCR technique,and provide a technical support for the quarative and quantitive detection ofkefeng No.6rice.Primers and probes were designed according to the sequence exogenous gene and border sequence and used for thekefeng No.6detection and other agriculture products.Results indicated that this detection method is specific forkefeng No.6GM rice.The relative sensitivity of this method is 0.01%.Therefor... |
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| Keywords: | "kefeng No 6" Real-time PCR Quantitative detection |
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