| 蛋白质组学研究中的新技术 |
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| 引用本文： | 王阳梦,何聪芬,董银卯.蛋白质组学研究中的新技术[J].生物技术通报,2005(5):46-50. |
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| 作者姓名： | 王阳梦 何聪芬 董银卯 |
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| 作者单位： | 北京工商大学北京市植物资源研究开发重点实验室 北京100037
(王阳梦，何聪芬)，北京工商大学北京市植物资源研究开发重点实验室 北京100037(董银卯) |
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| 摘 要： | 随着后基因组时代的到来,蛋白质组研究日益受到密切关注。蛋白质组学的发展需要有先进的技术平台作支撑。除了蛋白质组研究中的三大核心技术-双向电泳、质谱和生物信息学技术,近几年又不断涌现出许多新技术。本文对同位素标记亲和标签、酵母双杂交、多维色谱—质谱联用等新技术作一概述。
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| 关 键 词： | 蛋白质组 同位素标记亲和标签技术 酵母双杂交技术 串联亲和纯化技术 多维色谱—质谱联用技术 蛋白质芯片技术 |
| 收稿时间： | 2005-06-27 |
| 修稿时间： | 2005年6月27日 |
Review of Some new Technologies for Proteomic Research |
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| Wang Yangmeng, He Congfen, Dong Yinmao.Review of Some new Technologies for Proteomic Research[J].Biotechnology Bulletin,2005(5):46-50. |
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| Authors: | Wang Yangmeng He Congfen Dong Yinmao |
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| Institution: | Beijing Key lab of Plant Resources Research and Development, Beijing Technology and Business University,Beijing 100037 |
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| Abstract: | Proteomics is one of the most active resseach fields in the post-genomic era.It is generally acknowledged that the reseach of proteomics was based much on the development and improvement of techniques and methods.Nowadays,the three main techniques are two dimensional electrophoresis(2-DE),mass spectrometry(MS) and proteomic databases.Besides the three techniques,there are many new technologies come forth in the past few years.Here,some of the new technologies were summarized such as isotope-coded affinity tag(ICAT),yeast two hybrid system,multi-chromatography etc in this paper. |
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| Keywords: | Proteomics Isotope-coded affinity tag Yeast two-hybrid system Tandem affinity purification Multidimentional chromatography-MS Protein biochip |
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