| 甘肃现代肉羊新品种群羊GH基因外显子1多态性与体重关联分析 |
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| 引用本文： | 张浩,朱爱文,马友记,李发弟,陈国宏.甘肃现代肉羊新品种群羊GH基因外显子1多态性与体重关联分析[J].生物技术通报,2010(12). |
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| 作者姓名： | 张浩 朱爱文 马友记 李发弟 陈国宏 |
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| 作者单位： | 甘肃农业大学动物科学与技术学院,兰州,730070 |
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| 基金项目： | 甘肃省自然科学基金(096RJZA008); 甘肃省农业生物技术项目(GNSW-2007-04) |
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| 摘 要： | 根据GenBank发表的绵羊生长激素(GH)基因外显子1的序列设计一对引物,采用PCR-SSCP技术分析GH基因外显子1在甘肃现代肉羊新品种选育群羊中的单核苷酸多态性,并与3月龄前的体重进行关联分析。结果表明,GH基因外显子1在新品种群羊中存在多态性,检测到两种基因型(AA、AB),其301bp处有一个T/A突变和305bp处有一个G/A突变,初生重、1月、2月、3月龄体重AA、AB型都无显著性差异(P>0.05),但3月龄AB型个体的体重相对于AA型偏高,由此初步推断GH基因可能是影响绵羊体重性状的主基因或与主基因相连锁,可用以对绵羊体重性状进行标记辅助选择。
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| 关 键 词： | 生长激素基因 绵羊 PCR-SSCP 遗传多态性 体重 |
Polymorphism of Exon 1 of Growth Hormone Gene and Its Relationship with Body Weight of the Gansu Modern Meat Sheep New Breed Population |
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| Zhang Hao,Zhu Aiwen,Ma Youji,Li Fadi,Chen Guohong.Polymorphism of Exon 1 of Growth Hormone Gene and Its Relationship with Body Weight of the Gansu Modern Meat Sheep New Breed Population[J].Biotechnology Bulletin,2010(12). |
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| Authors: | Zhang Hao Zhu Aiwen Ma Youji Li Fadi Chen Guohong |
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| Institution: | Zhang Hao Zhu Aiwen Ma Youji Li Fadi Chen Guohong(College of Animal Science and Technology,Gansu Agricultural University,Lanzhou 730070) |
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| Abstract: | A pair of primers was designed according to the sequence of exon 1 of growth hormone(GH)gene published in GenBank.Single nucleotide polymorphism(SNPs)of exon 1 of GH gene was detected in Gansu modern meat sheep new breed population by polymerase chain reaction(PCR)-single strand conformation polymorphism(SSCP).The results showed that the polymorphism was detected and had two genotype(AA,AB)in Gansu modern meat sheep new breed population,which have a T/A mutation in 301 bp and G/A mutation in 305 bp of exon ... |
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| Keywords: | Growth hormone gene Sheep PCR-SSCP Genetic polymorphism Body weight |
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