Leptin介导JAK/STAT信号通路对猪皮下前脂肪细胞脂类代谢调控的研究

以猪原代皮下前脂肪细胞为研究材料,检测Leptin介导JAK/STAT信号通路中基因表达水平,旨在阐明Leptin介导JAK/STAT信号通路对脂肪代谢的分子机制。用0和100 ng/mL Leptin分别处理脂肪细胞48 h,油红O染色鉴定脂肪细胞,试剂盒测定细胞中甘油三酯和游离脂肪酸含量,Real-time PCR方法检测皮下前脂肪细胞中JAK/STAT信号通路中基因表达水平。研究结果表明:皮下前脂肪细胞中,Leptin组甘油三酯含量均显著低于对照组(P<0.05),脂肪酸含量均低于对照组;JAK/STAT信号通路中LepR、JAK2、STAT3、CPT-1、ACOX1和PGC-1α基因的表达量均显著高于对照组(P<0.05);LepR、JAK2、STAT3、CPT-1、ACOX1和PGC-1α基因表达量均与甘油三酯和游离脂肪酸含量呈显著负相关(P<0.05)。该研究结果表明,Leptin激活皮下前脂肪细胞中JAK/STAT信号通路,上调通路相关基因的表达,促进甘油三酯分解及脂肪酸氧化,降低细胞中甘油三酯和游离脂肪酸含量。

Study on the Regulation of Leptin-mediated JAK/STAT Signal Pathway on Lipid Metabolism in Porcine Subcutaneous Preadipocyte

Using in vitro cultured porcine subcutaneous preadipocytes as the study material,the expression levels of related genes were measured for investigating the molecular regulation mechanism of Leptin-mediate JAK/STAT signal pathway on lipid metabolism.The subcutaneous adipocytes were treated with 0 and 100 ng/mL Leptin for 48 h,and the dipocytes were identified by oil red O dyeing,and the contents of triglyceride and free fatty acids in the cells were measured by kit,and the mRNA expression levels of relate genes in the JAK/STAT signal pathway of subcutaneous preadipocytes were detected using real-time PCR technology.The results showed the content of the triglyceide of the subcutaneous preadipocyte that in Leptin group was significantly lower(P<0.05)than that in the control group,and the content of the free fatty acids was lower than that of the control group.The expression levels of gene lepR,JAK2,STAT3,CPT-1,ACOX1,and PGC-1 in JAK/STAT signal pathway in Leptin group were significantly higher than that in control group(P<0.05).The expression levels of gene lepR,JAK2,STAT3,CPT-1,ACOX1,and PGC-1 were significantly negatively correlated with the content of triglyceride and free fatty acid(P<0.05).In conclusion,the results suggested that Leptin activated JAK/STAT signal pathway in subcutaneous preadipocyte,increased the mRNA expressions of relevant genes in the JAK/STAT signaling pathway,promoted the oxidation of fatty acid and the decomposition of triglyceride,and thus decreased the content of triglycerides and free fatty acids in subcutaneous preadipocyte.