| 鼠肝中金属硫蛋白的纯化 |
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| 引用本文： | 王雪,赵海峰,吕明,李鸿雁,吴文杰,刘娅,李正强.鼠肝中金属硫蛋白的纯化[J].生物技术通报,2010(3). |
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| 作者姓名： | 王雪 赵海峰 吕明 李鸿雁 吴文杰 刘娅 李正强 |
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| 作者单位： | 吉林大学,长春,130021 |
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| 摘 要： | 旨在探索鼠肝中金属硫蛋白(MT)提取工艺并加以改进。按照经典方法工艺提取MT,用中空纤维柱超滤方法加以改进,依据MT自身特点进行分离和鉴定。结果显示,粗品符合MT特点:分子量在6.5kD左右;无280nm特征吸收峰,加酸后紫外吸收(200-300nm)肩峰消失;通过离子交换可以实现MT1、MT2的分离;通过原子吸收测定,含有锌、铜、镉3种金属,锌含量最高,具有重要的病理生理意义。因此,与其它方法比较,改进后方法更适宜实验室制备。
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| 关 键 词： | 金属硫蛋白 大鼠 肝脏 层析 |
The Purification Technology Metallothionein in Rat Liver |
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| Wang Xue,Zhao Haifeng,Lv Ming,Li Hongyan,Wu Wenjie,Liu Ya,Li Zhengqiang.The Purification Technology Metallothionein in Rat Liver[J].Biotechnology Bulletin,2010(3). |
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| Authors: | Wang Xue Zhao Haifeng Lv Ming Li Hongyan Wu Wenjie Liu Ya Li Zhengqiang |
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| Institution: | Wang Xue Zhao Haifeng Lv Ming Li Hongyan Wu Wenjie Liu Ya Li Zhengqiang(Jilin University,Changchun 130021) |
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| Abstract: | It was to purify the metallothionein from the livers of mature rats by typical procedures and make some improvements.MT was isolated by using traditional method which was improved hollow-fiber ultrafiltration.Results showed that the extraction of protein molecular weight was around 6.5 kD,without 280 nm peak and the absorption shoulder disappeared(200-300 nm)when acid added.The crude protein could be separated the two forms through DEAE chromatography.The atomic absorption analysis showed that the protein c... |
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| Keywords: | Metallothionein Rat Liver Chromatography |
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