甲壳动物血液凝固的分子机制

甲壳动物的防御机制完全依赖于非特异性免疫系统,血淋巴的凝固在宿主防御和阻止血淋巴渗漏中起重要的作用.甲壳动物的凝固酶的激活有两种独立的方式.在鳌虾中,依赖于Ca2+的转谷氨酰胺酶催化可溶性的凝固蛋白原转变成共价交联的凝固蛋白多聚物,不需要蛋白裂解反应.相反,在鲎中,位于淋巴细胞中凝固反应的所有因子都在受到脂多糖激活分泌到胞外,一系列的蛋白裂解反应使得凝固蛋白原转变成凝固蛋白,凝固蛋白间通过非共价的交联结合,而有转谷氨酰胺酶催化的凝固蛋白和淋巴细胞表面proxin间的结合则更加稳定了凝固蛋白反应.

Molecular Mechanism of Coagulation in Crustacean

The defense mechanisms of crustaceans depend completely on the innate immune system,hemolymph coagulation is very important for the host defense and for prevention of leakage of hemolymph.Proclotting enzyme can be activated by two independent pathways in Crustacea.In crayfish,soluble coagulogen are converted into covalently crosslinked polymers of coagulin by Ca~(2+)-dependent transglutaminase,but without the proteolytic cascade.In contrast,all horseshoe crab coagulation factors are stored in the granules o...