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| 农杆菌介导法向玉米茎尖导入抗草甘膦EPSPS基因的研究 | |
| 引用本文： | 孙传波,李海华,郭嘉,陶蕊,曲文利,孟凡梅,张举仁,刘文国,袁英.农杆菌介导法向玉米茎尖导入抗草甘膦EPSPS基因的研究[J].生物技术通报,2011(3). |
| 作者姓名： | 孙传波 李海华 郭嘉 陶蕊 曲文利 孟凡梅 张举仁 刘文国 袁英 |
| 作者单位： | 1. 吉林省农业科学院生物技术研究中心,长春,130033 2. 山东大学生命科学学院,济南,250100 3. 吉林省农业科学院玉米所,长春,130033 |
| 基金项目： | 抗逆转基因玉米新品种培育项目(2009ZX08003-018B) |
| 摘 要： | 以玉米自交系郑58的茎尖为受体,通过农杆菌介导法将抗草甘膦EPSPS基因转入玉米中,研究以茎尖为受体的农杆菌转化体系的可行性。98株转化苗,经过300 ppm的除草剂(农达)筛选,共获得13株转基因植株,经PCR检测,其中7株表现阳性,转化率达7.14%。初步证明外源基因已经整合到玉米基因组中,以玉米茎尖作为受体的转化系统用于基因转化是可行、高效的。 |
| 关 键 词： | 玉米 茎尖 农杆菌介导 EPSPS基因 |
Study on Agrobacterium tumefaciens Mediated Transformation of EPSPS Gene into Shoot Apical Point of Maize |
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| Sun Chuanbo,Li Haihua,Guo Jia,Tao Rui,Qu Wenli,Meng Fanmei,Zhang Juren,Liu Wenguo,Yuan Ying.Study on Agrobacterium tumefaciens Mediated Transformation of EPSPS Gene into Shoot Apical Point of Maize[J].Biotechnology Bulletin,2011(3). | |
| Authors: | Sun Chuanbo Li Haihua Guo Jia Tao Rui Qu Wenli Meng Fanmei Zhang Juren Liu Wenguo Yuan Ying |
| Institution: | Sun Chuanbo1 Li Haihua1 Guo Jia1 Tao Rui1 Qu Wenli1 Meng Fanmei1 Zhang Juren2 Liu Wenguo3 Yuan Ying1(1Biotechnology Research Centre,Jilin Academy of Agricultural Sciences,Changchun 130033,2College of Life Science,Shandong University,Jinan 250100,3Maize Research Institute,Changchun 130033) |
| Abstract: | The EPSPS gene was transferred into the shoot apical point of maize inbred line zheng 58 by Agrobacterium tumefaciens mediated transformation to study the feasibility of this system.13 transgenic plants were obtained after selecting with 300 ppm(nong da)and 7 of them were confirmed positive by PCR amplification.The frequency of transformation reached about 7.14%.This study showed that the foreign gene was integrated into maize genome and the shoot apical point of maize as receptor in transformation was feas... |
| Keywords: | Maize Shoot apical point Agrobacterium tumefaciens mediated EPSPS gene |
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