| 稀有海洋放线菌Salinispora arenicola非核糖体肽合成酶和卤代酶生物合成基因簇核心区的克隆及序列分析 |
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| 引用本文： | 马艳玲,邓海,魏菁菁,郭秀红,刘中来,邓灵福,刘艳丽,郭建军,姚汉超,熊国梅,祁超.稀有海洋放线菌Salinispora arenicola非核糖体肽合成酶和卤代酶生物合成基因簇核心区的克隆及序列分析[J].生物技术通报,2011(2). |
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| 作者姓名： | 马艳玲 邓海 魏菁菁 郭秀红 刘中来 邓灵福 刘艳丽 郭建军 姚汉超 熊国梅 祁超 |
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| 作者单位： | 华中师范大学生命科学学院,遗传调控与整合生物学湖北省重点实验室,武汉,430079 |
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| 基金项目： | 国家自然科学基金资助项目(20772040); 湖北省自然基金资助项目(2009CDB234); 遗传调控与整合生物学湖北省重点实验室开放项目(200904) |
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| 摘 要： | 克隆稀有海洋放线菌Salinispora arenicola的非核糖体肽合成酶(NRPS)和卤代酶生物合成基因簇核心区基因片段。根据已发表的放线菌NRPS和卤代酶生物合成基因簇核心区的核苷酸序列保守区设计两对简并性引物,采用PCR的方法扩增NRPS和卤代酶生物合成基因簇核心区基因片段,使用分子生物学软件进行序列分析。获得两段大小分别为662bp和557bp的基因片段,编码220个和185个氨基酸。这两段序列与海洋放线菌Salinispora arenicola CNS-205的NRPS和卤代酶生物合成基因簇核心区基因核苷酸序列的同源性分别为99%和98%。成功地获得了稀有海洋放线菌Salinispora arenicola的NRPS和卤代酶生物合成基因簇核心区基因片段,该基因片段的获取将为分离全长基因簇以及研究该基因簇在生物合成中的功能奠定基础。
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| 关 键 词： | Salinispora arenicola 腺苷酰化结构域 色氨酸卤代酶 系统进化 |
Cloning and Sequences Analysis of Core Regions of NRPS and Halogenase Gene Cluster from Rare Marine Actinomycete Salinispora arenicola |
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| Ma Yanling,Deng Hai,Wei Jingjing,Guo Xiuhong,Liu Zhonglai,Deng Lingfu,Liu Yanli,Guo Jianjun,Yao Hanchao,Xiong Guomei,Qi Chao.Cloning and Sequences Analysis of Core Regions of NRPS and Halogenase Gene Cluster from Rare Marine Actinomycete Salinispora arenicola[J].Biotechnology Bulletin,2011(2). |
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| Authors: | Ma Yanling Deng Hai Wei Jingjing Guo Xiuhong Liu Zhonglai Deng Lingfu Liu Yanli Guo Jianjun Yao Hanchao Xiong Guomei Qi Chao |
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| Institution: | Ma Yanling Deng Hai Wei Jingjing Guo Xiuhong Liu Zhonglai Deng LingfuLiu Yanli Guo Jianjun Yao Hanchao Xiong Guomei Qi Chao(Hubei Key Laboratory of Genetic Regulation and Integrative Biology,College of Life Sciences,Huazhong Normal University,Wuhan 430079) |
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| Abstract: | To obtain the fragments related to NRPS and Halogenase gene cluster,degenerate primers were designed based on the conserved sequences of NRPS and halide gene cluster from other microbes.The NRPS and Halogenase gene fragmentwas obtained by PCR,and molecular biological softwares were used to analyze homologies.The NRPS and Halogenase gene cluster fragment contains 662 bp and 557 bp nucleotide acids,encoding 220 and 185 amino acids,respectively.Homology comparisons with NRPS and Halogenase gene sequences from ... |
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| Keywords: | Salinispora arenicola Adenylation domain Tryptophan halogenase Phylogenetic |
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