| 高表达精脒/精胺N1-乙酞基转移酶对人肺癌A549细胞株生长的影响 |
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| 引用本文： | 柳蔚,韩钰,蔡富强,王艳林.高表达精脒/精胺N1-乙酞基转移酶对人肺癌A549细胞株生长的影响[J].生物技术通报,2011(3). |
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| 作者姓名： | 柳蔚 韩钰 蔡富强 王艳林 |
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| 作者单位： | 三峡大学分子生物学研究所三峡大学医学院,宜昌,443002 |
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| 基金项目： | 国家自然科学基金资助项目(30772590); 湖北省教育厅重点研究资助项目(D200713003) |
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| 摘 要： | 旨在研究人精脒/精胺N1-乙酰基转移酶(spermidine/spermine N1-acetyltransferase,SSAT)高表达对人肺癌A549细胞生长的影响。以pCR2.1-SSAT质粒为模板,PCR法扩增人SSAT基因并克隆至pcDNA3.1表达载体。重组质粒转染A549细胞后,RT-PCR法和Western blotting法筛选SSAT高表达的细胞株。MTT法检测细胞增殖,流式细胞仪检测细胞周期。成功构建pcDNA3.1-SSAT重组质粒,用该质粒转染A549细胞后,筛选获得稳定高表达SSAT的细胞株。SSAT高表达导致细胞生长抑制,S期细胞减少和自发性凋亡细胞增多。结果显示,稳定高表达SSAT可在A549肺癌细胞中部分模拟多胺类似物类抗癌药物的药理活性,导致瘤细胞生长抑制和细胞凋亡。
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| 关 键 词： | SSAT 真核表达 肺癌细胞 |
Effects of High Expression of Spermidine/Spermine N1-Acetyltransferase on the Growth of Human A549 Lung Cancer Cell Line |
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| Liu Wei,Han Yu,Cai Fuqiang,Wang Yanlin.Effects of High Expression of Spermidine/Spermine N1-Acetyltransferase on the Growth of Human A549 Lung Cancer Cell Line[J].Biotechnology Bulletin,2011(3). |
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| Authors: | Liu Wei Han Yu Cai Fuqiang Wang Yanlin |
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| Institution: | Liu Wei Han Yu Cai Fuqiang Wang Yanlin(Institute of Molecular Biology,Medical College,Three Gorges University,Yichang 443002) |
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| Abstract: | It was to study the effects of high expression of spermidine/spermine N1-acetyltransferase(SSAT)on the growth of A549 human lung cancer cells.Human SSAT cDNA was amplified by PCR from pCR2.1-SSAT plasmid and then cloned into pcDNA3.1 vector.After this recombinant plasmid was transfected into A549 cells,the cell lines with SSAT high expression were selected by RT-PCR and Western blotting assay.Cell growth was assayed using MTT method and the cell cycle was analyzed via flow cytometry.The eukaryotic expressio... |
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| Keywords: | SSAT Eukaryotic expression Lung cancer cell |
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