| 四种黄单胞菌的基因芯片检测方法的建立 |
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| 引用本文： | 龙海,李一农,李芳荣.四种黄单胞菌的基因芯片检测方法的建立[J].生物技术通报,2011(1). |
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| 作者姓名： | 龙海 李一农 李芳荣 |
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| 作者单位： | 1. 深圳出入境检验检疫局,深圳,518001;深圳市检验检疫科学研究院,深圳,518045
2. 深圳出入境检验检疫局,深圳,518001 |
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| 基金项目： | 深圳出入境检验检疫局博士基金项目(SZ2007101) |
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| 摘 要： | 结合双重PCR和基因芯片技术同时检测和鉴定我国检疫性细菌,包括水稻白叶枯病菌(Xanthomonas oryzae pv.oryzae,Xoo)、水稻细菌性条斑病菌(X.oryzae pv.oryzicola,Xooc)、柑桔溃疡病菌(X.axonopodis pv.citri,Xac)以及严重危害十字花科作物的甘蓝黑腐病菌(Xanthomonas campestris pv.campestris,Xcc)。以铁载体受体(Putative siderophore receptor)基因序列和RNA多聚酶西格玛因子(RNA polymerase sigma factor,rpoD)基因序列为靶标,设计引物和特异性探针能够同时检测这4种重要的病原菌。对17个细菌菌株进行芯片检测,仅4种靶标菌得到阳性结果,证明此方法具有很高的特异性。4种致病菌基因组DNA的检测灵敏度约为3 pg。检测结果表明,建立的基因芯片检测方法特异性强,能实现上述4种黄单胞菌的准确检测和鉴定,具有良好的应用前景。
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| 关 键 词： | 黄单胞菌 双重PCR 基因芯片 检测 |
Detection of Four Xanthomonas by Gene Chip |
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| Long Hai,Li Yinong,Li Fangrong.Detection of Four Xanthomonas by Gene Chip[J].Biotechnology Bulletin,2011(1). |
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| Authors: | Long Hai Li Yinong Li Fangrong |
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| Institution: | Long Hai1,2 Li Yinong1 Li Fangrong1(1Shenzhen Entry-Exit Inspection and Quarantine Bureau,Shenzhen 518001,2 Shenzhen Academy of Inspection and Quarantine,Shenzhen 518045) |
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| Abstract: | A specific,rapid and sensitive method of gene chip combined with double PCR allow the simultaneous detection of Xanthomonas oryzae pv.Oryzae,X.oryzae pv.Oryzicola,X.axonopodis pv.citri and X.campestris pv.campestris.The Putative siderophore receptor gene and RNA polymerase sigma factor(rpoD)was selected to design primers and species-specific probes which can simultaneously detect these four kinds of bacteria.Genomic DNAs from 17 bacterial strains were detected and only 4 index bacteria were positive.The det... |
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| Keywords: | Xanthomonas Double PCR Gene chip Detection |
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