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| 中国荷斯坦牛白细胞黏附缺陷症PCR-RFLP检测方法的研究 | |
| 引用本文： | 王洪梅,李建斌,许尚忠,高运东,张秀红,刘文浩,仲跻峰.中国荷斯坦牛白细胞黏附缺陷症PCR-RFLP检测方法的研究[J].生物技术通报,2007,0(3):155-158,170. |
| 作者姓名： | 王洪梅 李建斌 许尚忠 高运东 张秀红 刘文浩 仲跻峰 |
| 作者单位： | 1. 山东省农业科学院奶牛研究中心,济南,250100 2. 中国农业科学院北京畜牧兽医研究所,北京,100094 3. 山东奥克斯生物技术有限公司,济南,250100 |
| 基金项目： | 山东省农业良种产业化工程项目;山东省农业科学院青年基金 |
| 摘 要： | 本试验根据已知牛染色体上CD18编码基因序列设计引物,提取牛血液和精液DNA,可扩增出338bp的DNA片段,将PCR产物克隆到pMD18-T载体中,对阳性重组质粒进行测序,确定为牛的CD18基因。由于CD18基因的383位碱基由A变为G,而引起牛白细胞黏附缺陷症(BLAD),通过对济南市11个奶牛场356头奶牛及53头荷斯坦种公牛进行了BLAD的PCR-RFLP检测,共检出3头杂合母牛(携带者),占检测母牛群的0.84%,在荷斯坦公牛中只检测到一种基因型,没有发现隐性突变基因的携带者。 |
| 关 键 词： | 荷斯坦牛 牛白细胞黏附缺陷症 测序 |
| 修稿时间： | 2007-01-22 |
Development of PCR-RFLP for Detecting Leuocyte Adhesion Deficiency in Chinese Holstein Cattle |
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| Wang Hongmei,Li Jianbin,Xu Shangzhong,Gao Yundong,Zhang Xiuhong,Liu Wenhao,Zhong Jifeng.Development of PCR-RFLP for Detecting Leuocyte Adhesion Deficiency in Chinese Holstein Cattle[J].Biotechnology Bulletin,2007,0(3):155-158,170. | |
| Authors: | Wang Hongmei Li Jianbin Xu Shangzhong Gao Yundong Zhang Xiuhong Liu Wenhao Zhong Jifeng |
| Institution: | 1Dairy Cattle Center of Shandong Academy of Agricultural Sciences ,yinan 250100;2Institute of Animal Sciences,Chinese Agricultural Sciences ,Beijing 100094 ; 3Shandong Ox Biotechnology CO, , Jinan 250100 |
| Abstract: | One pair of primers were synthesized according to the alien published the gene encoding bovine CD18.Exteacting DNA from blood and semen,A 338bp sequence was amplified by PCR and cloned into the pMD18-T Vector respectively.The positive clones of CD18 gene were identified by sequence analysis.One point mutation at A383G was identified within the gene encoding bovine CD18 in a Holstein calf afflicted with BLAD.The samples from 356 Holstein dairy cow in 11 herds and 53 Holstein bulls in Jinan were examined by PCR-RFLP.Three heterozygotoes(BLAD-carrier)were detected from Chinese Holstein dairy cattles.The BLAD-carrier prevalence in Holstein dairy cattle was estimated to be 0.84%.Only wild genotype was found in Chinese Holstein bulls,without the recessive BLAD gene. |
| Keywords: | PCR-RFLP |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |