四引物扩增受阻突变体系PCR技术及其在动植物遗传育种研究中的应用

四引物扩增受阻突变体系PCR(Tetra-primer amplification refractory mutation system PCR,Tetra-primer ARMS PCR)技术是一种在普通PCR基础上发展起来的单核苷酸多态性(SNP)分型技术。该项技术综合了扩增受阻突变体系(Amplification refractory mutation system,ARMS)和四引物PCR(tetra-primer PCR)技术的优点,是对等位基因特异性PCR法的改良。它具有操作简便、分型快速、费用低廉等特点,在国内外生命科学领域尤其是遗传育种领域的应用越来越广泛。本文介绍了四引物扩增受阻突变体系PCR的技术原理及优势、结果检测手段和反应体系改进方法,并在此基础上对该技术在遗传育种研究中的应用进行综述。

Tetra-primer Amplification Refractory Mutation System PCR and Its Application in Fauna and Flora Genetics and Breeding Research

Tetra-primer amplification refractory mutation system PCR(tetra-primer ARMS PCR)is a technique of typing SNP developed on the basis of common PCR.This technique combines the advantages of amplification refractory mutation system(ARMS)and four-primer PCR(tetra-primer PCR),is an improvement of allele specific PCR.It has the characteristics of simple operation,rapid typing and low cost and has been applied more and more widely in the field of life sciences especially in the field of breeding.In this paper,the principle and advantages of tetra-primer ARMS PCR technique,detection methods of results and improvement methods of reaction system are introduced.In addition,the applications of this technique in genetics and breeding researches are reviewed.