| 不同处理方法对海稻米中γ-氨基丁酸提取率的影响 |
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| 引用本文： | 杨悦,高郡茹,杨柳.不同处理方法对海稻米中γ-氨基丁酸提取率的影响[J].生物技术通报,2020,26(3):38-41. |
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| 作者姓名： | 杨悦 高郡茹 杨柳 |
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| 作者单位： | （1广东医科大学广东天然药物研究与开发重点试验室，广东湛江524023；2广东医科大学海洋医药研究院，广东湛江524023；3广东海洋大学食品学院，广东湛江524088）
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| 基金项目： | 国家自然科学基金项目(31560319);广西高校大学生创新训练项目(201810602063) |
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| 摘 要： | 以海稻米为研究对象，研究提取温度、提取溶剂、料液比、提取时间、提取次数等5个因素对海稻米中γ 氨基丁酸（GABA）提取率的影响，采用正交试验分析方法确定海稻米中GABA最优工艺条件。结果表明：海稻米中GABA的最佳提取工艺为：提取溶剂为水、提取时间为1 h、提取次数3次、提取温度60 ℃、提取物料比1 g∶15 mL，在此提取条件下的提取率为6.2μg/g。
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| 关 键 词： | 海稻米 &gamma 氨基丁酸 提取率 优化 |
Research Progress on CRISPR Technology in Biology and Medical Science |
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| YANG Yue,GAO Jun-ru,YANG Liu.Research Progress on CRISPR Technology in Biology and Medical Science[J].Biotechnology Bulletin,2020,26(3):38-41. |
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| Authors: | YANG Yue GAO Jun-ru YANG Liu |
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| Institution: | (Research Center for Biomedical Sciences,Guangxi Normal University,Guilin 541004;Guangxi Universities Key Laboratory of Stem cell and Biopharmaceutical Technology,Guilin 541004;College of Sciences,Guangxi Normal University,Guilin 541004) |
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| Abstract: | As an emerging technology,CRISPR has been developing rapidly in recent years,and there are great research achievements in disease detection,as well as in the construction of model organisms and gene therapy field.As its simple principle,easy to operate and it can be combined with a variety of technologies,CRISPR technology has great potential in medicine,agriculture,environment and other fields.In addition to the classic CRISPR/cas9 system,scientists have discovered various other types CRISPR/Cas system.Meanwhile,the single base editor(BE)based on Cas9 protein and the CRISPR/Cas13a system that can edit RNA also greatly expand the application range of CRISPR.This article will review the latest development of CRISPR technology and its application in biology,medicine and other fields. |
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| Keywords: | CRISPR/Cas9 single base editor RNA editing gene therapy |
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