CRISPR /Cas9基因编辑技术在山羊和绵羊中的应用研究进展

CRISPR/Cas9系统是近年来新兴的一种基因编辑技术,可将特定DNA基因序列敲除、插入或定点突变,具有快捷、高效、精准、特异性高等特点,广泛地应用于遗传育种、生物医药和基因工程等研究领域。山羊和绵羊是重要的经济家畜动物和实验动物,利用CRISPR/Cas9基因编辑系统对羊进行遗传修饰,将加速品种改良,提高动物生产性能,获得更加优质的农副产品。主要对CRISPR/Cas9基因编辑技术的概述、作用机理及在羊乳"人源化"改造、提高肉品质、改善毛纤维质量等方面的应用研究进展及发展前景作简要阐述,以期为相关科研人员提供参考。

Research Progress of CRISPR/Cas9 Gene Editing Technology in Goat and Sheep

CRISPR/cas9 system is a new gene editing technology in recent years,and it can precisely edit and modify the specific DNA sequence of organism,including knockout,insertion or site-directed mutation.It is simple,efficient,precise and specific.Therefore,it has been widely used in biomedicine,gene engineering and genetic breeding research.The goat and sheep are important economic domestic animal and experimental animal.Genetic modification of sheep and goat by CRISPR/cas9 editing system can accelerate breed improvement,increase livestock production and obtain high quality agricultural and sideline products.Here we mainly summarize the overview,mechanism and the research progress of CRISPR/cas9 gene editing technology and its application in“humanized”modification of goat milk,raising meat quality,improving wool fiber quality,and the prospect,aiming at providing reference for relevant researchers.