赤松枯梢病叶内生真菌多样性研究

在微生物层面研究枯梢病与植物针叶内生微生物的互作关系,分析内生真菌的多样性差异,为松枯梢病的防控提供基础数据。利用高通量测序技术测定赤松(Pinus densiflora)不同染病程度的针叶内生真菌的多样性。结果显示,随着病害的加重,P. densiflora针叶内生真菌丰富度呈现出上升的趋势,多样性指数表现为先下降后上升的趋势。无病斑针叶内生真菌中,子囊菌门(Ascomycota)与担子菌门(Basidiomycota)相对丰度最高,优势属为Lapidomyces和Selenophoma,病害导致优势菌相对丰度的降低。通过对不同染病情况的P. densiflora针叶内生真菌的测定,明确了枯梢病不同发病程度的P. densiflora针叶内生真菌的多样性及群落结构组成。

Endophytic Fungi Diversity in the Needles of Pinus densiflora with Sphaeropsis sapinea

The objective of this work is to study the interaction between shoot blight of pine and endophytic fungi in needles at the microbial level and to analyze the community structure and diversity of endophytic fungi for providing basic data in the prevention of shoot blight of pine. High-throughput sequencing technology was used to determine the diversity and community structure of endophytic fungi in different level of blight of P. densiflora. Results demonstrated that the richness of endophytic fungi in the P. densiflora needles showed an upward trend,and the diversity index tended of decreasing first and then increasing. Ascomycota and Basidiomycota were phyla of the highest relative abundance in the asymptomatic needles,and the dominant species of endophytic fungi were Lapidomyces and Selenophoma. The disease caused the relative abundance of dominant species to decrease. In conclusion,the determination of fungi in the needles of P. densiflora clarified the diversity and community structure of endophytic fungi in the needles of P. densiflora with different levels of S. sapinea.