丢糟和磷矿粉高温堆肥中耐高温解磷菌的筛选及性能分析

为了解决高温极端环境下的磷素溶出问题,从高温堆肥中分离出具有耐高温能力的解磷微生物。该研究利用无机磷选择培养基,从添加磷矿粉和丢糟的高温堆肥样品中,分离筛选出5株耐高温解磷菌(细菌为GDB1-2;真菌为GDF1-3),并对这5株菌株进行形态学和分子生物学鉴定及解磷能力分析。研究结果表明,筛选获得的解磷菌株分别为枯草芽孢杆菌(Bacillus subtilis)、地衣芽孢杆菌(Bacillus licheniformis)、多枝横梗霉(Lichtheimia ramosa)、烟曲霉(Aspergillus fumigatus)及构巢曲霉(Aspergillus nidulans)。该5株菌具有较好的耐高温和耐pH性能,各菌株耐高温范围为40-60℃,在50℃条件下其解磷能力均达到最大值,当初始pH 在5-9范围时各菌株均能保持一定的解磷能力。此外,通过解磷曲线发现各菌株的最高解磷量范围在136.85-174.33 μg/mL。该研究结果为后续开发高温环境微生物资源提供了素材,具有良好的应用推广前景。

Screening and Performance Analysis of High-temperature Phosphate-solubilizing Microorgams in High Temperature Compost with Phosphate Rock and Discarded Vinasse Lost Grains

In order to solve the problem of the phosphorus dissolution in the high temperature environment,the phosphate-solubilizing microorgams were screened from high temperature compost.Using inorganic phosphorus selective medium,5 strains of high temperature resistant and phosphate-solubilizing microorganisms(bacteria GDB1-2;fungus GDF1-3)were isolated and screened from the high-temperature compost samples added with phosphate rock and lost grains.The 5 strains were identified by morphological and molecular biology and solubilized-phosphorus capability.The results showed that the 5 isolates were Bacillus subtilis,Bacillus licheniformis,Lichtheimia ramosa,Aspergillus fumigatus and Aspergillus nidulans.The phosphorus solubilization curve of the 5 strains showed that the highest phosphorus content of each strain ranged from 136.85 to 174.33 μg/mL.It was found that each strain had a wide range of high temperature resistance and grew between 40-60℃,but its ability to dissolve phosphorus reached a maximum at 50℃.Under the initial pH of 5-9,all the 5 strains maintained a certain ability of dissolving phosphorus.The research results provide materials for the subsequent development of high-temperature environmental microbial resources,and have a good application and promotion prospects.