基因功能研究的加速器-高通量克隆表达系统

大规模基因测序已经完成了多个重要物种的基因组序列分析,功能基因组学研究的序幕已经拉开。大规模的体外克隆获得全长或部分的开放阅读框是蛋白质表达、干扰分析和定位分析、相互作用分析的必经环节,对使用简便、成本低廉、保真度高和易于在不同体系中穿梭的高通量克隆体系的了解、评价和使用已经成为基因组水平研究的重要问题。为此,对目前存在的多种高通量克隆体系进行了系统的介绍和比较。

The Accelerator of Functional Research-High-Throughput Gene Cloning and Expression Systems

Genomes of a dozen of important species have been completed sequenced,which heralded a new era of functional genomics.Cloning full-length or partial open reading frames is the critical step for protein expression,interfering analysis,localization,interaction component study.A high-throughput cloning system with easy to use,low cost,high fidelity and shuttle between different expression systems conveniently is very important for genome-wide research and so to understand,evaluate and use.The principal and applications of cloning system currently available were introduced and compared.