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| SDS-CTAB结合法提取棉花总DNA | |
| 引用本文： | 孙鑫,崔洪志,胡宝忠,郭三堆.SDS-CTAB结合法提取棉花总DNA[J].生物技术通报,2004(5):45-47. |
| 作者姓名： | 孙鑫 崔洪志 胡宝忠 郭三堆 |
| 作者单位： | 1. 中国农业科学院生物技术研究所,北京,100081;东北农业大学生命科学学院,哈尔滨,150030 2. 中国农业科学院生物技术研究所,北京,100081 3. 东北农业大学生命科学学院,哈尔滨,150030 |
| 摘 要： | 根据以往提取棉花总DNA的经验 ,并参考了几种相关的植物总DNA提取方法 ,得到一种更适合棉花 (GossypiumL .)总DNA提取的方法。该提取方法综合了SDS法与CTAB法的优点 ,使获得的棉花总DNA纯度高 ,得率大 ,完整性较好 ,可用于PCR检测 ,Southern杂交 ,RAPD ,染色体步移等分子生物学操作。 |
| 关 键 词： | 棉花 总DNA SDS CTAB |
A SDS-CTAB Combined Method for Extracting Total DNA from Cotton Plant |
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| Sun Xin,Chui Hongzhi,Hu Baozhong,Guo Sandui.A SDS-CTAB Combined Method for Extracting Total DNA from Cotton Plant[J].Biotechnology Bulletin,2004(5):45-47. | |
| Authors: | Sun Xin Chui Hongzhi Hu Baozhong Guo Sandui |
| Institution: | Sun Xin2 Chui Hongzhi1 Hu Baozhong2 Guo Sandui1 |
| Abstract: | We reported a improved method based on the former experience and according to several method relative to isolation of total DNA. The cotton total DNA obtained by the method which combines SDS method and CTAB method, showed to be more integral, pure and heavy producing. It is suitable for PCR, Southern blotting, RAPD, Genome Walking and other molecular biology operation. |
| Keywords: | Cotton Total DNA SDS CTAB |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |