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| GH79家族糖苷水解酶分子进化关系和蛋白结构研究 | |
| 引用本文： | 陈泉冰,曹伟洁,李春,吕波.GH79家族糖苷水解酶分子进化关系和蛋白结构研究[J].生物技术通报,2023,39(1):104-114. |
| 作者姓名： | 陈泉冰 曹伟洁 李春 吕波 |
| 作者单位： | 1.北京理工大学化学与化工学院生物化工研究所医药分子科学与制剂工程工信部重点实验室，北京 102488 2.清华大学化学工程系，北京 100084 |
| 基金项目： | 国家重点研发计划(2018YFA0901800);天津合成生物技术创新能力提升项目(TSBICIP-CYFH-002) |
| 摘 要： | GH79家族的糖苷水解酶在碳水化合物改性、细胞免疫识别和信号传导等方面具有广泛的生理活性和重要的应用前景。然而，目前GH79家族的多样性催化机理仍不清楚，识别底物的结构基础和分子机制尚不清晰。本文总结了近几年GH79家族的研究进展，系统分析了GH79家族酶的来源与分布，通过对酶的序列特征、分子进化关系、蛋白结构解析等方面进行深入阐述，旨在为后续的GH79家族的蛋白质工程和功能催化机制的解析奠定基础。 |
| 关 键 词： | GH79家族 催化机理 分子进化 结构解析 |
| 收稿时间： | 2022-04-07 |
Molecular Evolutionary Relationship and Protein Structure of Glycoside Hydrolases from GH79 Family |
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| CHEN Quan-bing,CAO Wei-jie,LI Chun,LV Bo.Molecular Evolutionary Relationship and Protein Structure of Glycoside Hydrolases from GH79 Family[J].Biotechnology Bulletin,2023,39(1):104-114. | |
| Authors: | CHEN Quan-bing CAO Wei-jie LI Chun LV Bo |
| Institution: | 1. Key Laboratory of Medical Molecule Science and Pharmaceutics Engineering, Ministry of Industry and Information Technology, Institute of Biochemical Engineering, School of Chemistry and Chemical Engineering, Beijing Institute of Technology, Beijing 102488 2. Department of Chemical Engineering, Tsinghua University, Beijing 100084 |
| Abstract: | The glycoside hydrolases of the GH79 family have extensive physiological activities and important application prospects in carbohydrate modification, cellular immune recognition and signal transduction. However, the diverse catalysis mechanism of GH79 family remains unclear, and the structural basis and molecular mechanism of substrate recognition are still unclear. This review summarizes the research progress of the GH79 family, systematically analyzes the origin and distribution of GH79 family enzymes, and deeply expounds their sequence features, molecular evolutionary relationships, and the crystal structures analysis, aiming to lay a foundation for protein engineering and functional metabolic mechanism of GH79 family. |
| Keywords: | GH family 79 catalytic mechanism molecular evolution structural analysis |
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