| 乙肝相关性肝癌中新基因CHCHD2的生物信息学分析 |
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| 引用本文： | 姚杨,苏杰,刘凯歌,徐锐,成碧萍.乙肝相关性肝癌中新基因CHCHD2的生物信息学分析[J].生物技术通报,2012,0(3):179-185. |
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| 作者姓名： | 姚杨 苏杰 刘凯歌 徐锐 成碧萍 |
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| 作者单位： | 西安医学院附属医院,西安,710077 |
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| 基金项目： | 西安医学院附属医院基金项目 |
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| 摘 要： | 应用基因芯片技术获取以稳定转染HBx基因的肝癌细胞HepG2(HepG2-X)及非转染的肝癌细胞HepG2的差异表达的基因,利用生物信息学方法对新基因CHCHD2进行初步分析表明,该蛋白开放性读码框长456 bp,编码151个氨基酸残基,相对分子量为15.55 kD,等电点9.43,是主要定位于线粒体中的亲水性蛋白,二级结构均以α-螺旋和无规则卷曲为主要构件。同源性比较分析结果表明,其碱基序列与已经报道的其他17个物种相似性为64%-99%,且符合种属之间的进化关系。
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| 关 键 词： | 乙肝相关性肝癌 基因芯片 CHCHD2 |
Bioinformatic Analysis of A Novel Gene CHCHD2 in Hepatocellular Carcinoma |
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| Yao Yang
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Su Jie
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Liu Kaige
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Xu Rui
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Cheng Biping.Bioinformatic Analysis of A Novel Gene CHCHD2 in Hepatocellular Carcinoma[J].Biotechnology Bulletin,2012,0(3):179-185. |
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| Authors: | Yao Yang Su Jie Liu Kaige Xu Rui Cheng Biping |
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| Institution: | Yao Yang Su Jie Liu Kaige Xu Rui Cheng Biping(The First Affiliated Hospital of Xi’an Medical University,Xi’an 710077) |
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| Abstract: | It was to obtain differentially expressed genes related to human hepatocellular carcinoma by cDNA microarr-ray technology.An initial bioinformatic analysis was performed on a novel gene,CHCHD2,which contains an ORF of 456 base pairs and encodes a polypeptide of 151 residues with a predicted molecular weight about 15.55 kD and pI9.43.CHCHD2 were mainly distributed in the mitochondria,is a hydrophilic protein.Alpha helix and random coil are main components of all secondary structures,and shows 64%-99% identity with the others species. |
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| Keywords: | HBV-related hepatocellular carcinoma cDNA microarr-ray technology CHCHD2 |
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