| 荔枝DNA提取及RAPD扩增条件优化 |
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| 引用本文： | 尤有利,王江波,施维属,潘东明.荔枝DNA提取及RAPD扩增条件优化[J].生物技术通报,2010(4). |
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| 作者姓名： | 尤有利 王江波 施维属 潘东明 |
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| 作者单位： | 1. 福建永春县农业技术推广站,泉州,362600
2. 福建农林大学园艺学院,福建农林大学园艺产品贮运保鲜研究所,福州,350002 |
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| 基金项目： | 泉州市科技计划项目，我国台湾农业新品种、新技术引进创新研究与示范项目，我国台湾果树新品种与品质控制新技术引进创新研究项目 |
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| 摘 要： | 为应用RAPD技术开展对荔枝种质资源的分析,以S43(GTCGCCGTCA)为引物,通过试验设计,分别研究了退火温度、模板浓度、引物浓度、dNTP浓度、Taq DNA聚合酶用量对荔枝RAPD-PCR反应的影响。建立并优化了适宜荔枝RAPD分析的扩增体系:20μL的反应体系,30ng的模板DNA度,0.25μmol/LRAPD引物、1.0UTaqDNA聚合酶,0.2μmol/LdNTP为荔枝适宜的RAPD-PCR扩增条件。
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| 关 键 词： | 荔枝 优化 |
Extration of Genomic DNA of Litchi chinensis and Optimizationof the RAPD Reaction System |
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| You Youli,Wang Jiangbo,Shi Weishu,Pan Dongming.Extration of Genomic DNA of Litchi chinensis and Optimizationof the RAPD Reaction System[J].Biotechnology Bulletin,2010(4). |
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| Authors: | You Youli Wang Jiangbo Shi Weishu Pan Dongming |
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| Institution: | You Youli1Wang Jiangbo2Shi Weishu2Pan Dongming2(1 Yongchun Agro-Techinical Extension Station,Quanzhou 362600,2College of Horticulture,Fujian Agriculture , Forestry University,Institute of Storage Science , Technology ofHorticultural Products,Fuzhou 350002) |
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| Abstract: | In order to use RAPD markers to analyze the resources in Litchi,the annealing temperature, template,concentration of primer, dNTP and the Taq DNA polymerase dosage on RAPD-PCR applications were tested to determine their optimal level . Besides,the following optimal reaction system for RAPD analysis in Litchi were established,including PCR reaction volume of 20 μL,30 ng DNA template,0.25 μmol/L RAPD primer,1.0 U Taq DNA polymerase,and 0.2 μmol/L dNTP in each reaction system of 20 μL. |
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| Keywords: | RAPD PCR Litchi chinensis Sonn RAPD Optimize PCR |
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