加工产品中转基因玉米Bt11成分实时荧光PCR定量(性)检测

实验在玉米自身基因和外源基因的边界序列之间设计了具有品种和品系特异性的引物和探针 ,并以实时荧光PCR技术 ,建立了加工产品中转基因玉米Bt1 1成分品系鉴定检测和定量检测的方法。实验对加热条件和时间对检测转基因成分的影响作了探讨 ,并检测了部分市售食品和饲料。检测结果发现 ,加热时间温度越高、时间越长 ,对转基因成分定量检测的影响越大 ;在所检测的样品中可以检测出转基因玉米Bt1 1成分 ,有些样品还同时检出其他转基因成分。本研究实验建立的方法 ,可以用于加工产品中转基因成分的定量检测 ,也可以用于定性检测 ,或作为常规PCR定性检测后的确证实验方法。

Quantitative/Identified Detection of the Genetically Modified Maize Bt11 Components in Processed Products

A quantitative/identified method was developed for detecting genetically modified (GM) maize Bt11 components in foods and in feeds by real time PCR. The event specific primer and probe set overlapping the junction was designed and used for assay. The effect of heat treatment on the detection of the genes by quantitative PCR methods was investigated. The Results revealed that the measure of inserted genes was affected by temperature and different time intervals of heat treatment. For further understanding of whether the commercially available processed maize materials mixed with GM maize, the samples of foods and feeds were collected from market and detected by the real time PCR methods developed in this study. Results showed that both foods and feeds could be detected GM maize Bt11 components.