| 康氏木霉基因组DNA提取方法的比较研究 |
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| 引用本文： | 张晓晖,郭春华,江晓霞,罗虹.康氏木霉基因组DNA提取方法的比较研究[J].生物技术通报,2007,0(5):128-130. |
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| 作者姓名： | 张晓晖 郭春华 江晓霞 罗虹 |
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| 作者单位： | 西南民族大学生命科学与技术学院,成都,610041 |
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| 摘 要： | 采用3种常规DNA提取方法提取瑞氏木霉基因组DNA。结果表明:冷冻研磨CTAB法更适合此真菌DNA的提取,试剂盒提取的总DNA纯度和浓度也较高,但是价格昂贵,饱和酚抽提法提取的DNA浓度不高,且易有降解现象,不适合分子生物学操作的要求。
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| 关 键 词： | 康氏木霉 基因组DNA 饱和酚抽提法 冷冻研磨CTAB法 试剂盒法 |
| 修稿时间： | 2007-03-27 |
Studies on Extraction of Genomic DNA from Trichoderma koningii |
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| Zhang Xiaohui,Guo Chunhua,Jiang Xiaoxia,Luo Hong.Studies on Extraction of Genomic DNA from Trichoderma koningii[J].Biotechnology Bulletin,2007,0(5):128-130. |
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| Authors: | Zhang Xiaohui Guo Chunhua Jiang Xiaoxia Luo Hong |
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| Institution: | Department of life science and technology,southwest university for nationalities,Chengdu 610041 |
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| Abstract: | Genomic DNA for future molecule-biology research was extracted from trichoderma Koningii by three methods.The results show that:Refrigeration-rubbing CTAB method is suitable for extraction of genomic DNA from Triehoderma Koningii.Kit method can get DNA template with higher purity and yield,but it is very expensive.The method of Liquid nitrogen grind-ss-pheno extraction gets a low yield of DNA and is not suitable for molecule-biology experiments. |
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| Keywords: | Triehoderma koningii Genomie DNA SS-Pheno extraction method Refrigeration-rubbing CTAB method Kit method |
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