| shRNA抑制人卵巢癌SW626细胞CXCR4基因的研究 |
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| 引用本文： | 汤容,于国新,袁瑞.shRNA抑制人卵巢癌SW626细胞CXCR4基因的研究[J].生物技术通报,2010(4). |
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| 作者姓名： | 汤容 于国新 袁瑞 |
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| 作者单位： | 重庆医科大学附属第一医院妇产科,重庆,400016 |
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| 基金项目： | 重庆市自然科学基金计划项目 |
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| 摘 要： | 探讨应用RNA干扰技术沉默趋化因子受体4(CXCR4)基因的表达,研究其对人卵巢癌SW626细胞增殖的抑制作用。设计合成两对特异性针对CXCR4基因的Oligo siRNA,用脂质体转染法转染至SW626细胞中,荧光共聚焦显微镜检测转染效率,采用Western blotting检测CXCR4蛋白表达情况,同时利用M'IT试验检测转染后细胞增殖抑制情况。结果显示,转染Oligo siRNA后,SW626细胞CXCR4蛋白表达水平降低(P0.05);细胞增殖的抑制率明显增高(P0.05)。体外合成的特异性针对CXCR4基因的Oligo siRNA对卵巢癌细胞株SW626中CXCR4基因表达和细胞增殖均有明显抑制作用。
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| 关 键 词： | 小干扰RNA CXCR4基因 卵巢癌 增殖 |
Influence of RNA Interference on Expression of CXCR4 in Human Ovarian Cancer Cells |
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| Tang Rong,Yu Guoxin,Yuan Rui.Influence of RNA Interference on Expression of CXCR4 in Human Ovarian Cancer Cells[J].Biotechnology Bulletin,2010(4). |
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| Authors: | Tang Rong Yu Guoxin Yuan Rui |
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| Institution: | Tang RongYu Guoxin Yuan Rui (Department of Obstetrics , Gynecology,the First Affiliated Hospital of Chongqing Medical University,Chongqing 400016) |
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| Abstract: | It was to investigate the inhibitory effect of RNA interference on the expression of CXCR4 in human ovarian cancer cells.Two pairs of oligo small interference RNA(Oligo siRNA)specific for CXCR4 were designed for RNAi and were transferred into SW626 cells by using Lipofectamine TM 2000,the transfection efficiency was observed.The expression of CXCR4 were detected by Western blotting.Furthermore,the cell proliferation was observed by MTT after transfection.Results showed that after transfection with Oligo siR... |
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| Keywords: | RNA interference CXCR4 gene Ovarian cancer Proliferation |
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