| 高效氯氰菊酯降解菌CH7的分离鉴定及降解条件的优化 |
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| 引用本文： | 张琛,王圣惠,闫艳春.高效氯氰菊酯降解菌CH7的分离鉴定及降解条件的优化[J].生物技术通报,2010(1). |
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| 作者姓名： | 张琛 王圣惠 闫艳春 |
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| 作者单位： | 1. 山东农业大学生命科学学院,泰安,271018
2. 中国农业科学院研究生院,北京,100081 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 从农药厂活性污泥中,分离到一株能以高效氯氰菊酯为唯一碳源生长的细菌CH7。经生理生化试验和16S rD-NA分析,将菌株CH7鉴定为铜绿假单胞菌(Pseudomonas aeruginosa)。采用Box-behnken设计试验、响应面法(response surfacemethodology)优化菌株CH7的降解条件。在最优条件下(29.4°C,pH7.0,接种量0.15g/L),菌株CH7在12d内对100mg/L高效氯氰菊酯的降解率为90%。
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| 关 键 词： | 高效氯氰菊酯 铜绿假单胞菌 响应面法 降解条件优化 |
Isolation,Characterization and Condition Optimization of Beta-cypermethrin Degrading Strain CH7 |
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| Zhang Chen,Wang Shenghui,Yan Yanchun.Isolation,Characterization and Condition Optimization of Beta-cypermethrin Degrading Strain CH7[J].Biotechnology Bulletin,2010(1). |
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| Authors: | Zhang Chen Wang Shenghui Yan Yanchun |
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| Institution: | Zhang Chen1 Wang Shenghui2 Yan Yanchun2(1College of Life Science of Sh,ong Agricultural University,Taian 271018,2Graduate School of Chinese Academy of Agricultural Sciences,Beijing 100081) |
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| Abstract: | Strain CH7,isolated from activated sludge,could utilize beta-cypermethrin as the sole carbon resource for growth.Based on the results of physiological-biochemical properties and phylogenetic similarity of 16S rDNA gene sequences,strain CH7 was identified as Pseudomonas aeruginosa.Response surface methodology based on the Box-behnken design of experiment was used to optimize the degrading conditions of strain CH7.Under the optimum degrading conditions(29.4 C,pH7.0,biomass 0.15 g/L),strain CH7 could degrade 9... |
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| Keywords: | Beta-cypermethrin Pseudomonas aeruginosa Response surface methodology Degrading condition optimization |
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