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| 刺参基因组DNA提取的探讨 | |
| 引用本文： | 孙孝德,孙国华,杨建敏,吉成龙,王卫军,宋志乐.刺参基因组DNA提取的探讨[J].生物技术通报,2010(3). |
| 作者姓名： | 孙孝德 孙国华 杨建敏 吉成龙 王卫军 宋志乐 |
| 作者单位： | 1. 上海海洋大学水产与生命学院,上海,201306;山东省海洋水产研究所,烟台,264006 2. 山东省海洋水产研究所,烟台,264006 3. 烟台市芝罘区渔业技术推广站,烟台,264000 |
| 基金项目： | 山东省博士基金项目，山东省科技发展计划项目，山东省良种工程项目 |
| 摘 要： | 以刺参为试验材料,分别以CTAB法、SDS法、Segama试剂盒法对刺参的触手、管足、体壁、纵肌、肠和呼吸树组织进行基因组DNA的提取,均得到了高质量的基因组DNA。Segama试剂盒提取DNA条带较其他两种方法清晰,杂质少且无降解,效果最佳。对比6种不同的刺参组织,其中纵肌组织3种方法均获得高质量基因组DNA,为提取基因组DNA的首选组织。开发了利用刺参触手和管足活体取样提取基因组DNA的方法,得到了高质量的基因组DNA,这为刺参无损伤取样提供了数据支持,使得将来刺参家系建立过程中保证亲体的健康存活及减少试验样品取样所带来的损伤成为可能。 |
| 关 键 词： | 刺参 基因组DNA DNA提取 无损伤取样 CTAB法 SDS法 Segama试剂盒法 |
Studies on Genomic DNA Extraction of Sea Cucumbers(Stichopus japonicus Selenka) |
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| Sun Xiaode,Sun Guohua,Yang Jianmin,Ji Chenglong,Wang Weijun,Song Zhile.Studies on Genomic DNA Extraction of Sea Cucumbers(Stichopus japonicus Selenka)[J].Biotechnology Bulletin,2010(3). | |
| Authors: | Sun Xiaode Sun Guohua Yang Jianmin Ji Chenglong Wang Weijun Song Zhile |
| Institution: | Sun Xiaode1,2 Sun Guohua2 Yang Jianmin2 Ji Chenglong1,2 Wang Weijun2 Song Zhile3(1College of Fisheries , Life Science of Shanghai Ocean University,Shanghai 201306,2Marine Fisheries Research Institute of Sh,ong Province,Yantai 264006,3Fisheries Technical Extension Station of the Zhifu District of Yantai City,Yantai 264000) |
| Abstract: | Genomic DNA of different tissues from Stichopus japonicus was extracted through CTAB method,SDS method and Segama kit method,respectively.High quality genomic DNA was isolated from six kinds of sea cucumbers tissues,including tube feet,body wall,longitudinal muscles,intestinal and respiratory tree.Compare to the other two methods,Segama kit got clearer DNA band without degradation.The best quality of genomic DNA from Longitudinal muscle tissue of Apostichopus japonicus was obtained by three methods.At the s... |
| Keywords: | Sea cucumbers Genomic DNA DNA extraction Non-invasive sampling CTAB method SDS method Segama kit method |
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