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| 转几丁质酶基因黑杨的获得 | |
| 引用本文： | 孟亮,李红双,金德敏,崔德才,王斌.转几丁质酶基因黑杨的获得[J].生物技术通报,2004(3):48-51. |
| 作者姓名： | 孟亮 李红双 金德敏 崔德才 王斌 |
| 作者单位： | 1. 山东农业大学生命科学学院,泰安,271018 2. 山东农业大学应用化学学院,泰安,271018 3. 中国科学院遗传与发育研究所,北京,100101 |
| 摘 要： | 以G1黑杨幼叶为外植体建立了组织培养高频再生体系 ,对传统的农杆菌介导法进行了改进 ,用来自于菜豆的几丁质酶基因 (CH5B)进行转化 ,经PCR和PCR Southern杂交鉴定 ,证实CH5B基因已整合入G1黑杨核基因组中。 |
| 关 键 词： | 黑杨 根癌农杆菌 转化 几丁质酶基因 转基因杨树 |
Transformation of Populus deltoids with CH5B gene |
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| Meng Liang,Li Hongshuang,Jin Demin,Cui Decai,Wang Bin.Transformation of Populus deltoids with CH5B gene[J].Biotechnology Bulletin,2004(3):48-51. | |
| Authors: | Meng Liang Li Hongshuang Jin Demin Cui Decai Wang Bin |
| Institution: | Meng Liang1 Li Hongshuang2 Jin Demin3 Cui Decai1 Wang Bin3 |
| Abstract: | The high frequency regeneration system of Populus deltoids, G1 was established in this study. The CH5B (chtinase 5B) gene was introduced by modified Agrobacterium tumefaciens mediated method into G1. PCR and PCR-Southern tests showed that CH5B gene has been integrated into Populus deltoids genome. |
| Keywords: | Populus deltoids Agrobacterium tumefaciens Transformation Chitinase gene Transgenic Populus deltoids |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |