基因组细菌人工染色体文库(BAC)的构建及应用

细菌人工染色体 (BAC)是一种承载DNA大片段的克隆载体系统 ,用于人、动物和植物基因组文库构建。BAC具有插入片断大、嵌合率低、遗传稳定性好、易于操作等优点。BAC文库的构建是基因组较大的真核生物基因组学研究的重要基础 ,可用于真核生物重要基因及全基因组物理作图、重要性状基因的图位克隆、基因结构及功能分析。本文主要综述了细菌人工染色体的构建与其鉴定 ,及其在物理图谱构建、图位克隆、转基因技术等研究上的应用。

Construction and Application of Genomics Bacterial Artificial Chromosome (BAC) Library

Bacterial artificial chromosome (BAC) is a kind of vector system used to construct large fragment insert libraries of genome DNA in human, animals and plants. Bacterial artificial chromosome has several notable advantages, such as inserting large fragments, maintaining the stability of inserted DNA in E.coli, producing few chrisms, recovering inserted DNA from E.coli cell and easy in library construction. Bacterial artificial chromosome libraries have some important purposes in the research of eukaryote genomes. They have been used for physical mapping of eukaryote genomes, map-based cloning of agronomically important trait genes and gene structure and function study. This paper presents a brief introduction of the development of BAC, and the process of construction, identification and application of BAC libraries.