| cDNA sequence analysis of ribosomal protein S13 gene in Plutella xylostella （Lepidoptera： Plutellidae） |
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| 作者姓名： | SHAO-LIWANG CHENG-FASHENG CHUAN-LINGQIAO MIYATATADASHI |
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| 作者单位： | [1]StateKeyLaboratoryofIntegratedManagementofPestInsectsandRodents,InstituteofZoology,ChineseAcademyofSciences,Beijing,China. [2]LaboratoryofAppliedEntomology,GraduateSchoolofBioagriculturalSciences,NagoyaUniversity,Nagoya,Japan |
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| 摘 要： | Ribosomal protein S 13 gene has been cloned and analyzed in many organisms,but there are few documents relating to insects. In this communication, the full-length cDNA sequence of ribosomal protein S 13 gene in the diamondback moth, Plutella xylostella(Lepidoptera: Plutellidae), was determined by using PCR amplification technique. The features of the ribosomal protein S 13 gene sequence were analyzed and the deduced amino acids sequence was compared with those from other insects. The results of multi-alignment of the amino acid sequences between the diamondback moth and other insect species revealed that this gene sequence is highly conserved in insects. Based on maximum likelihood method, a phylogenetic tree was constructed from 10 different species using PHYLIP software. It showed that nematode is one separate lineage and the five insect speciesbe long to another lineage, whereas those species higher than insects form the third one. The pattern of this phylogenetic tree evidently represented the evolution of different species.
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| 关 键 词： | cDNA序列分析 核糖体蛋白S13 Plutella xylostella 鳞翅目 菜蛾科 分子鉴定 |
cDNA sequence analysis of ribosomal protein S13 gene in Plutella xylostella (Lepidoptera: Plutellidae) |
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| Authors: | SHAO-LI WANG CHENG-FA SHENG CHUAN-LING QIAO MIYATA TADASHI |
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| Institution: | State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China;, Laboratory of Applied Entomology, Graduate School of Bio agricultural Sciences, Nagoya University, Nagoya, Japan |
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| Abstract: | Abstract Ribosomal protein S13 gene has been cloned and analyzed in many organisms, but there are few documents relating to insects. In this communication, the full-length cDNA sequence of ribosomal protein S13 gene in the diamondback moth, Plutella xylostella (Lepidoptera: Plutellidae), was determined by using PCR amplification technique. The features of the ribosomal protein S13 gene sequence were analyzed and the deduced amino acids sequence was compared with those from other insects. The results of multi-alignment of the amino acid sequences between the diamondback moth and other insect species revealed that this gene sequence is highly conserved in insects. Based on maximum likelihood method, a phylogenetic tree was constructed from 10 different species using PHYLIP software. It showed that nematode is one separate lineage and the five insect species belong to another lineage, whereas those species higher than insects form the third one. The pattern of this phylogenetic tree evidently represented the evolution of different species. |
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| Keywords: | cDNA sequence identity analysis Plutella xylostella ribosomal protein S13 |
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