| Partial genomic organization of ribosomal protein S7 gene from malaria vector Anopheles stephensi |
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| 作者姓名： | RAJNIKANT DIXIT SARITA DIXIT UPAL ROY YOGESH S.SHOUCHE SURENDRA GAKHAR |
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| 作者单位： | [1]Department of Biosciences, Maharshi Dayanand University, Rohtak,Pune University Campus, Pune, India [2]National Centre for Cell Science, Pune University Campus, Pune, India |
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| 基金项目： | Acknowledgments This work was supported by DST New Delhi. The authors are thankful to Dr S.K. Subbarao and Dr T. Aadak, Malaria Research Centre, Delhi, for providing the mosquitoes. |
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| 摘 要： | In this study, we describe the partial genomic organization of ribosomal protein S7 gene isolated from the mosquito Anopheles stephensi. Initially a 558 bp partial cDNA sequence was amplified as precursor mRNA sequence containing 223 bp long intron. 5＇ and 3＇ end sequences were recovered using end specific rapid amplification of cDNA ends （RACE） polymerase chain reaction. The full-length cDNA sequence was 914 nucleotide long with an open reading frame capable of encoding 192 amino acid long protein with calculated molecular mass of 22174 Da and a pI point of 9.94. Protein homology search revealed 〉75% identity to other insect＇s S7 ribosomal proteins. Analysis of sequence alignment revealed several highly conserved domains, one of which is related to nuclear localization signal （NLS） region of human rpS7. Interestingly, intron nucleotide sequence comparison with A. gambiae showed a lesser degree of conservation as compared to coding and untranslated regions. Like this, early studies on the genomic organization and cDNA/ Expressed sequence tag analysis （EST） could help in genome annotation ofA. stephensi, and would be likely to be sequenced in the future.
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| 关 键 词： | 疟疾媒介昆虫 斯氏按蚊 核醣体蛋白S7基因 基因组结构 |
| 修稿时间： | 2006-11-11 |
Partial genomic organization of ribosomal protein S7 gene from malaria vector Anopheles stephensi |
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| Authors: | RAJNIKANT DIXIT SARITA DIXIT UPAL ROY YOGESH S SHOUCHE SURENDRA GAKHAR |
| |
| Institution: | Department of Biosciences, Maharshi Dayanand University, Rohtak, India;National Centre for Cell Science, Pune University Campus, Pune, India |
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| Abstract: | In this study, we describe the partial genomic organization of ribosomal protein S7 gene isolated from the mosquito Anopheles stephensi. Initially a 558 bp partial cDNA sequence was amplified as precursor mRNA sequence containing 223 bp long intron. 5′ and 3′ end sequences were recovered using end specific rapid amplification of cDNA ends (RACE) polymerase chain reaction. The full‐length cDNA sequence was 914 nucleotide long with an open reading frame capable of encoding 192 amino acid long protein with calculated molecular mass of 22 174 Da and a pI point of 9.94. Protein homology search revealed > 75% identity to other insect's S7 ribosomal proteins. Analysis of sequence alignment revealed several highly conserved domains, one of which is related to nuclear localization signal (NLS) region of human rpS7. Interestingly, intron nucleotide sequence comparison with A. gambiae showed a lesser degree of conservation as compared to coding and untranslated regions. Like this, early studies on the genomic organization and cDNA/ Expressed sequence tag analysis (EST) could help in genome annotation of A. stephensi, and would be likely to be sequenced in the future. |
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| Keywords: | Anopheles malaria mosquito ribosomal protein vector |
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