白介素1β时间依赖性地抑制大鼠皮层神经元钾电流

白细胞介素1β（Interleukin-1β，IL-1β）是一种重要的促炎细胞因子，在中枢神经系统中发挥着广泛的生物学功能。大量研究表明，IL-1β的作用非常复杂，在不同的模型和条件下作用不同，包括神经损伤或者神经毒性作用。电压门控钾通道调节神经元电学性质，也参与多种中枢神经系统的病理学过程。虽然IL-1β和钾通道都在脑损伤和脑疾病过程中发挥重要作用，但目前还很少有它们之间关系的研究报导。文章作者以原代培养的大鼠皮层神经元为材料，使用全细胞膜片钳技术，研究了10 ng/mL的IL-1β在不同处理时间下对皮层神经元电压依赖性钾电流的影响。根据电流的性质，可以将记录到的钾电流分为瞬时外向电流（IA）为主的IA样电流和延迟整流电流（IK）为主的IK样电流两部分，结果显示：IL-1β处理8 h对二者没有作用；处理24 h可使IA样和IK样电流的幅度降低20%左右。以上结果提示IL-1β对大鼠皮层神经元电压门控钾电流具有抑制作用，并且这种抑制可能具有时间依赖性。

Potassium Currents in Neurons of Rats Were Reduced by IL-1β in Time Dependent Manner

Interleukin-1β (IL-1β) is an important porinflammatory cytokine that exert widely biology functions in central nervous system (CNS). Many Studies had reported that the effects of IL-1β were complicated. These effects include both of neuroprotective and neurotoxic effects in different models or conditions. Voltage-gated K+ channels contribute to the excitability of neurons, and regulate shape of action potential. Although both of IL-1β and K+ channels play key roles in injury and disease of brain, so far the relations of them was reported in few studies. In this study, whole cell patch-clamp recording was used to investigate the effects of IL-1β (10 ng/mL) treatment for 8 and 24 hours on voltage-dependent K+ currents of cultured cortical neurons from rats. Base on the properties of the currents, K+ currents were divided into two components, i.e. transient outward potassium current (IA)-dominant IA-like and delayed rectifier potassium current (IK)-dominant IK-like currents. The results showed that both of the K+ currents in cortical neurons were not affected by IL-1β treatment for 8 hours, but significantly inhibited by treatment for 24 hours. Our results suggested that the voltage-gated K+ currents were inhibited by IL-1β treatment in time dependent manner in cortical neurons of rats.