用光声光谱技术测量鼻咽癌细胞的光谱吸收特性

为了解人鼻咽癌细胞的光谱吸收特性,用双光束归一化光声光谱技术测量三株人鼻咽癌细胞(SUNE-1,CNE-2,CNE-1)的归一化吸收光谱和光谱吸收系数.结果表明(1)在波长4000～6000A范围内,这三株人鼻咽癌细胞的光谱吸收系数大小为SUNE-1＞CNE-2＞CNE-1;(2)在波长4200A处平均相对光声信号值为SUNE-11.36,CNE-21.20,CNE-11.10,三者之间的差异具有显著性(P值＝0.012).人鼻咽癌细胞株SUNE-1,CNE-2和CNE-1具有不同的光声光谱特性.

PHOTOACOUSTIC ABSORPTION CHARACTERISTICS OF SUNE-1, CNE-2,AND CNE-1 HUMAN NASOPHARYNGEAL CARCINOMA CELL LINESMEASURED USING PHOTOACOUSTIC TECHNIQUES

Purpose: To investigate optical absorption characteristics of human nasopharyngeal carcinoma cell lines. Methods: Standardized photoacoustic spectroscopy and optical absorption coefficient of SUNE-1,CNE-2,and CNE-1 were measured respectively using double beam standardized photoacoustic technique. Results:(1)in the range from 4000? to 6000?,optical absorption coefficient of three cell lines are in the following order: SUNE-1>CNE-2>CNE-1;(2)At wavelength 4200?, the mean values of relative photoacoustic signal for SUNE-1, CNE-2, and CNE-1 are 1.36, 1.20,and 1.10 respectively, showing a singnificant difference(p=0.012). Conclusion: Human nasopharyngeal carcinoma cell lines SUNE-1, CNE-2, and CNE-1 have different photoacoustic spectroscopic characteristics. This study lays a foundation of further research on biological behaviors of nasopharyngeal carcinoma.