剪切应力对毛细血管内皮细胞代谢的影响

建立的平行平板流协腔装置适用于研究血管内皮细胞代谢对剪切流场的响应。将培养的人胚肾小球血管单层内皮细胞置于剪应力分别为５＊１０＾－５Ｎ／ｃｍ＾２，１＊１０＾－４Ｎ／ｃｍ＾２和１．５＊１０＾－４Ｎ／ｃｍ＾２的定常层流中剪切２５小时。

EFFECT OF SHEAR STRESS ON METABOLISM BY CAPILLARY VASCULAR ENDOTHELIAL CELLS

A new parallel plate flow chamber apparatus has been established for the study of metabolic response of endothelial cells to a wide range of well-controlled steady and pulsatile shear stresses for long time periods. Cultured,replicate human glomerulus vascular endothelial cell monolayers were exposed to steady laminar shear stresses of 5×10-5 N/cm2,1×10-4 N/cm2 and 1.5×10-4 N/cm2 or in a stationary incubation for up to 25 hours, and the production rate and the cumulative production of endothelin were determined by radioimmunoassay. The results demonstrated that levels of shear stress affected significantly the metabolism of endothelin in HGVEC. Compared with the stationary incubation,exposure to such low levels of shear stresses as 5×10-5N/cm2 and 1×10-4N/cm2 may actually increase secretion of endothelin and that a higher level of wall shear stress of 1.5×10-4N/cm2 decreases the secretion. Effect of shear stress on the cumulative production of endothelin was greater than on the production rate of endothelin.