铜绿假单胞菌体外对肠上皮细胞骨架的影响

为探索铜绿假单胞菌粘附肠上皮细胞后，细胞骨架特性的变化规律及可能的机制。采用微管吸吮实验技术并结合细胞ELISA、图像分析等方法研究体外绿脓杆菌粘附肠上皮细胞后细胞骨架的变化。结果显示细菌粘附后1h肠上皮细胞活力即开始下降，3h后IEC面积明显减小，而细胞周长无明显变化；胞内骨架蛋白减少，且随孵育时间的延长愈趋明显；细胞弹性系数K1、K2在粘附后3h明显降低，同时伴有粘性系数μ也明显下降。以上结果表明绿脓杆菌粘附肠上皮细胞后，细胞骨架成分改变，细胞骨架功能受损害，最终导致细胞损伤。

EFFECTS OF Pseudomonas aeruginosa ADHESION ON CYTOSKELETON OF INTESTINAL EPITHELIAL CELLS

To explore the mechanism of intestinal epithelial cell(IEC) cytoskeleton injury after Pseudomonas aeruginosa adhesion,the micropipette aspiration technique and cell-ELISA, image analysis were adopted to investigate the changes of cytoskeleton protein for Pseudomonas aeruginosa adhesion. The result showed that the viability of the IEC was decreased at 1h, the area of IEC was significantly decreased, but the perimeter of IEC was unchanged before 3h. The increase of incubation time,the content of skeleton proteins were decreased more significantly. The elastic coefficient K₁,K₂ were markedly decreased, meanwhile the viscous coefficient μ was decreased. These results suggested that content of cytoskeleton changes, its function injured after Pseudomonas aeruginosa adhesion, finally leads to the damage of IEC.