| 用FTIR研究SDS对肌酸激酶二级结构的影响 |
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| 引用本文： | 张艳,陈培榕,周海梦.用FTIR研究SDS对肌酸激酶二级结构的影响[J].生物物理学报,1994,10(2):181-186. |
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| 作者姓名： | 张艳 陈培榕 周海梦 |
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| 作者单位： | 清华大学化学系，清华大学生物科学与技术系，中国科学院生物物理所生物大分子国家重点实验室 |
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| 基金项目： | 中关村地区联合分析测试基金 |
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| 摘 要： | 用去卷积付里叶红外光谱（ＦＴＩＲ）研究结果表明在低浓度的ＳＤＳ溶液中，二级结构各组份发生了明显可测的变化，表明酶的活性部位及其附近区域有较多的二级结构。
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| 关 键 词： | 肌酸激酶，十二烷基硫酸钠，变性，二级结构 |
THE EFFECT OF SDS CONCENTRATIONS ON THE SECONDARY STRUCTURE OF CREATINE KINASE STUDIED BY FTIR |
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| Zhang Yan,Chen Peirong.THE EFFECT OF SDS CONCENTRATIONS ON THE SECONDARY STRUCTURE OF CREATINE KINASE STUDIED BY FTIR[J].Acta Biophysica Sinica,1994,10(2):181-186. |
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| Authors: | Zhang Yan Chen Peirong |
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| Abstract: | The components of secondary strure of creatine kinase at the different concentrations of SDS have been examined by using Fourier transform infrared (FTIR) spectroscopy. The results show that the order secondary strucks of the enzyme at low concentration SDS decreased. It suggests that conformation at or near the active site contains more ordered secondary structure. |
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| Keywords: | Creatine kinase Sodium dodecyl sulfate DenaturationSecondary structure |
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