| 离子注入法将外源DNA直接导入小麦的研究 |
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| 引用本文： | 吴丽芳,尹若春,谷运红,吴李君,余增亮.离子注入法将外源DNA直接导入小麦的研究[J].生物物理学报,2001,17(4):724-730. |
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| 作者姓名： | 吴丽芳 尹若春 谷运红 吴李君 余增亮 |
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| 作者单位： | 1. 中国科学院等离子体物理研究所离子束生物工程学实验室,安徽,合肥,230031
2. 安徽大学生命科学学院生物技术系,安徽,合肥,230039 |
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| 基金项目： | 国家重点科技攻关项目(96-538 02-01)资助课题,安徽省自然科学基金资助项目(99047640) |
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| 摘 要： | 通过离子束介导法将外源GUS基因直接导入小麦成熟种子.组织化学染色结果表明GUS基因的瞬时表达率可以达到70%以上.当代(Ru)PCR分析结果表明,阳性植株的频率与离子注入剂量有关,适宜的注入剂量为7×106离子/cm2.PCR-Southern和Southern B1ot分析结果表明外源基因已整合到小麦基因组中,说明离子束介导外源DNA直接导入小麦是可行的.另外还探讨了用离子束介导创造小麦远缘分子杂种的可能性.
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| 关 键 词： | 离子注入 GUS基因 小麦 直接导入 成熟种子 外源DNA |
| 文章编号： | 1000-6737(2001)04-0724-07 |
| 修稿时间： | 2001年5月14日 |
STUDIES ON INTRODUCING EXOGENOUS DNA INTOWHEAT BY ION IMPLANTATION |
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| WU Li-fang,YIN Ruo-chun,GU Yun-hong,WU Li-jun,YU Zeng-liang.STUDIES ON INTRODUCING EXOGENOUS DNA INTOWHEAT BY ION IMPLANTATION[J].Acta Biophysica Sinica,2001,17(4):724-730. |
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| Authors: | WU Li-fang YIN Ruo-chun GU Yun-hong WU Li-jun YU Zeng-liang |
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| Institution: | WU Li-fang1,YIN Ruo-chun2,GU Yun-hong1,WU Li-jun1,YU Zeng-liang1 |
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| Abstract: | The exogenous GUS gene was introduced into wheat mature seeds by ion implantation. Histochemical staining showed the temporary expression rate of GUS gene reached more than 70%. PCR assays of R0 generation displayed that the positive plant rate was related to dosage of implanted ions, and the suitable dosage for transformation was 7×1016ions/cm2. Further PCR-Southern and Southern blot assays proved that exogenous gene had integrated into wheat genome. The experiments provided an effective method for DNA delivery into wheat and showed a good prospect of introducing exogenous genomic DNA with helpful gene into wheat. |
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| Keywords: | Ion implantation GUS gene Wheat Direct delivery Mature seeds |
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