人类基因组盒式外显子和内含子保留的可变剪接位点预测

信使RNA的可变剪接是真核生物有别于原核生物的基本特征之一，信使RNA前体的可变剪接极大地丰富了高等真核生物蛋白质的多样性，并与生物体的组织特异性密切相关。文章对人类盒式外显子和内含子保留的一些基本特征进行了统计；根据剪接位点附近的单碱基、碱基二联体和三联体的保守性等特征，利用基于多样性指标的二次判别法，对盒式外显子和内含子保留的供体端和受体端可变剪接位点进行了预测。交叉检验结果表明，盒式外显子供体端和受体端的识别精度分别达到93％、84％以上的水平；内含子保留供体端和受体端的识别精度分别达到89％、81％以上的水平。

PREDICTION OF ALTERNATIVE SPLICING SITES OF CASSETTE EXON AND INTRON RETENTION IN HUMAN GENOME

Alternative splicing of mRNA is a basilic distinguishing feature between enkaryotes and prokaryotes, and alternative splicing of premessenger RNA exponentially expands proteomic diversity, at the same time it relates with tissue-specific process of organism. Here, the authors analysed some basic features of cassette exon and intron retention in human genome; and based on the conservation of nucleotides, di-nucleotides, and tri-nucleotides at splice sites the method of increment of diversity with quadratic discriminant analysis was used to predict the donor and acceptor splice sites of cassette exon and intron retention. The results showed cassette exon's prediction accuracy of alternative donor and acceptor splice sites have attained higher than 93% and 84% respectively; intron retention's prediction accuracy of alternative donor and acceptor splice sites have attained 89% and 81% respectively.