没食子酸丙酯和没食子酸异丁酯对人红细胞膜结构与功能的影响

本文应用荧光探剂ANS(1—苯胺—8萘磺酸)、NPN(N—苯基—1—萘胺)和DPH(1.6—二苯基—1.3.5—已三烯)观察没食子酸丙醋和没食子酸异丁酯对人红细胞膜流动性和相变温度以及Na~ -K~ ATP酶活性的影响.实验结果指出该两种化合物均能:(1)降低与膜结合的荧光探剂强度但不改变探剂在水相与膜相的分配比例:(2)降低膜脂的相变温度,增加膜的流动性;(3)抑制红细胞膜Na~ -K~ ATP酶活性;(4)标记红细胞膜的DPH偏振度随化合物浓度的增加而降低,膜的流动性增加.在给定的浓度范围内,两种化合物的效应表现为明显的量效关系与构效关系.从上述结果推测该两种化合物可能是通过改变膜脂结构、膜蛋白的脂类环境而调节膜的功能,成为其治疗疾病的机理之一.

THE EFFECT OF PROPYL GALLATE AND ISO-BUTYL GALLATE ON STRUCTURE AND FUCTION OF HUMAN ERYTHROCYTE MEMBRANES

The effects of propyl gallate and iso-butyl gallate on the fluidity, phase transition and Na + -K+ ATP ase activity of human erythrocyte membranes have been studied using ANS, NPN and DPH probes as used. Results show that both compounds give rise to (1) a decrease of the fluorescence but no change in ANS partition from the aqueous medium to the bilayer; (2) a decrease of the phase transition temperature and an increase of fluidity of membranes; (3) a decrease of the Na + -K+ ATP ase activity of membranes; (4) a decrease of the fluorescence polarization of DPH labelled on the ghost and an increase of fluidity of membranes, as the concentration of both compounds increases. In the given region of concentration, the effect of propyl gallate and iso-butyl gallate shows quantitative-effective and structural-effective relationships.