Niosome-Encapsulated Gentamicin for Ophthalmic Controlled Delivery |
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Authors: | Ghada Abdelbary Nashwa El-gendy |
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Institution: | (1) Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Cairo University, Kaser el aini street, Cairo, Egypt;(2) Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Beni-Suef University, Beni-Suef, Egypt |
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Abstract: | The objective of the present research was to investigate the feasibility of using non-ionic surfactant vesicles (niosomes)
as carriers for the ophthalmic controlled delivery of a water soluble local antibiotic; gentamicin sulphate. Niosomal formulations
were prepared using various surfactants (Tween 60, Tween 80 or Brij 35), in the presence of cholesterol and a negative charge
inducer dicetyl phosphate (DCP) in different molar ratios and by employing a thin film hydration technique. The ability of
these vesicles to entrap the studied drug was evaluated by determining the entrapment efficiency %EE after centrifugation
and separation of the formed vesicles. Photomicroscopy and transmission electron microscopy as well as particle size analysis
were used to study the formation, morphology and size of the drug loaded niosomes. Results showed a substantial change in
the release rate and an alteration in the %EE of gentamicin sulphate from niosomal formulations upon varying type of surfactant,
cholesterol content and presence or absence of DCP. In-vitro drug release results confirmed that niosomal formulations have exhibited a high retention of gentamicin sulphate inside the
vesicles such that their in vitro release was slower compared to the drug solution. A preparation with 1:1:0.1 molar ratio of Tween 60, cholesterol and DCP
gave the most advantageous entrapment (92.02% ± 1.43) and release results (Q8h = 66.29% ± 1.33) as compared to other compositions. Ocular irritancy test performed on albino rabbits, showed no sign of
irritation for all tested niosomal formulations. |
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Keywords: | controlled release gentamicin sulphate niosomes ophthalmic |
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