Polyethylene Glycol on Stability of Chitosan Microparticulate Carrier for Protein |
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Authors: | Manee Luangtana-anan Sontaya Limmatvapirat Jurairat Nunthanid Rapeepun Chalongsuk Keiji Yamamoto |
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Institution: | (1) Department of Pharmaceutical Technology, Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, 73000, Thailand;(2) Pharmaceutical Biopolymer Group (PBiG), Faculty of Pharmacy, Silpakorn University, Nakhon Pathom, Thailand;(3) Department of Community Pharmacy, Faculty of Pharmacy, Silpakorn University, Nakorn Pathom, 73000, Thailand;(4) Department of Pharmaceutical Technology, Graduate School of Pharmaceutical Sciences, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan |
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Abstract: | Stability enhancement of protein-loaded chitosan microparticles under storage was investigated. Chitosan glutamate at 35 kDa
and bovine serum albumin as model protein drug were used in this study. The chitosan microparticles were prepared by ionotropic
gelation, and polyethylene glycol 200 (PEG 200) was applied after the formation of the particles. All chitosan microparticles
were kept at 25°C for 28 days. A comparison was made between those preparations with PEG 200 and without PEG 200. The changes
in the physicochemical properties of the microparticles such as size, zeta potential, pH, and percent loading capacity were
investigated after 0, 3, 7, 14, and 28 days of storage. It was found that the stability decreased upon storage and the aggregation
of microparticles could be observed for both preparations. The reduction in the zeta potential and the increase in the pH,
size, and loading capacity were observed when they were kept at a longer period. The significant change of those preparations
without PEG 200 was evident after 7 days of storage whereas those with PEG 200 underwent smaller changes with enhanced stability
after 28 days of storage. Therefore, this investigation gave valuable information on the stability enhancement of the microparticles.
Hence, enhanced stability of chitosan glutamate microparticles for the delivery of protein could be achieved by the application
of PEG 200. |
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