Increased serum heat-shock protein 70 levels reflect systemic inflammation, oxidative stress and hepatocellular injury in preeclampsia |
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Authors: | Attila Molvarec János Rigó Jr Levente Lázár Krisztián Balogh Veronika Makó László Cervenak Miklós Mézes Zoltán Prohászka |
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Institution: | 1st Department of Obstetrics and Gynecology, Semmelweis University, Budapest, Hungary. molvarec@freemail.hu |
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Abstract: | It has been previously reported that serum levels of 70-kDa heat-shock protein (Hsp70) are elevated in preeclampsia. The aim
of the present study was to examine whether increased serum Hsp70 levels are related to clinical characteristics and standard
laboratory parameters of preeclamptic patients, as well as to markers of inflammation (C-reactive protein), endothelial activation
(von Willebrand factor antigen) or endothelial injury (fibronectin), trophoblast debris (cell-free fetal DNA) and oxidative
stress (malondialdehyde). Sixty-seven preeclamptic patients and 70 normotensive, healthy pregnant women were involved in this
case-control study. Serum Hsp70 levels were measured with enzyme-linked immunosorbent assay (ELISA). Standard laboratory parameters
(clinical chemistry) and C-reactive protein (CRP) levels were determined by an autoanalyzer using the manufacturer’s kits.
Plasma von Willebrand factor antigen (VWF:Ag) levels were quantified by ELISA, and plasma fibronectin concentration by nephelometry.
The amount of cell-free fetal DNA in maternal plasma was determined by quantitative real-time polymerase chain reaction analysis
of the sex-determining region Y gene. Plasma malondialdehyde levels were measured by the thiobarbituric acid-based colorimetric
assay. Serum Hsp70 levels were increased in preeclampsia. Furthermore, serum levels of blood urea nitrogen, creatinine, bilirubin
and CRP, serum alanine aminotransferase and lactate dehydrogenase (LDH) activities, as well as plasma levels of VWF:Ag, fibronectin,
cell-free fetal DNA and malondialdehyde were also significantly higher in preeclamptic patients than in normotensive, healthy
pregnant women. In preeclamptic patients, serum Hsp70 levels showed significant correlations with serum CRP levels (Spearman
R = 0.32, p = 0.010), serum aspartate aminotransferase (R = 0.32, p = 0.008) and LDH activities (R = 0.50, p < 0.001), as well as with plasma malondialdehyde levels (R = 0.25, p = 0.043). However, there was no other relationship between serum Hsp70 levels and clinical characteristics (age, parity,
body mass index, blood pressure, gestational age, fetal birth weight) and laboratory parameters of preeclamptic patients,
including markers of endothelial activation or injury and trophoblast debris. In conclusion, increased serum Hsp70 levels
seem to reflect systemic inflammation, oxidative stress and hepatocellular injury in preeclampsia. Nevertheless, further studies
are required to determine whether circulating Hsp70 plays a causative role in the pathogenesis of the disease. |
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Keywords: | Biomarker Heat-shock protein 70 Hepatocellular injury Inflammation Oxidative stress Preeclampsia |
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