Comparative analysis of agglutinins from hemolymph and albumin gland ofHelix pomatia |
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Authors: | P Mullainadhan L Renwrantz |
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Institution: | (1) Zoologisches Institut und Zoologisches Museum der Universität Hamburg, Martin-Luther-King-Platz 3, D-2000 Hamburg 13, Germany |
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Abstract: | Summary The hemolymph ofHelix pomatia contains a weak agglutinating activity. This lectin concentration was calculated to be about 1.8 g·ml–1. Among the different red blood cells tested, pronase-treated sheep erythrocytes were found to be the most suitable indicator cells. Their agglutination could be inhibited by GalNAc and GlcNAc. The serum agglutinin was isolated by affinity chromatography using Sephadex G-200 as the matrix. It exhibited a single band in discontinuous PAGE. In the presence of SDS, subunits of 27000 daltons were obtained which, after addition of 2-mercaptoethanol, partly dissociated into 13000-dalton subunits. The biochemical properties observed were compared with those of the well-known blood group A-specific lectin from the albumin gland ofH. pomatia.Abbreviations
GalNac
N-acetyl-galactosamine
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GlcNAc
N-acetyl-glucosamine
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SDS
sodium dodecylsulfate |
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Keywords: | Lecithin ofHelix serum Hemolymph agglutinin Helix lecitins |
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