Hemolymph ion composition and volume changes in the supralittoral isopod Ligia pallasii Brandt, during molt |
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Authors: | A Ziegler T Grospietsch T H Carefoot J P Danko M Zimmer I Zerbst-Boroffka S C Pennings |
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Institution: | Z.E. Elektronenmikroskopie, University of Ulm, Germany. andreas.ziegler@medizin.uni-ulm.de |
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Abstract: | We analyzed ion composition and volume of the hemolymph of Ligia pallasii in four different stages of the molt cycle using capillary electrophoresis and 3H-inulin. The main ions in the hemolymph were Na+, K+, Mg2+, Ca2+, and Cl−. The Ca2+concentration increased significantly during the molt by 47% from intermolt to intramolt and by 37% from intermolt to postmolt,
probably due to resorption of Ca2+ from the cuticle and sternal CaCO3 deposits. The K+ concentration increased significantly by 20% during molt. The hemolymph volume normalized to the dry mass of the animals
decreased by 36% from intermolt to late premolt. This was due to a reduction in the hemolymph volume and to an increase in
dry mass of the animals during premolt. A sudden increase in the hemolymph volume occurring between late premolt and intramolt
served to expand the cuticle. Since the Na+, K+, Mg2+, and Cl− concentrations did not change significantly from late premolt to intramolt, the increase in hemolymph volume suggests an
uptake of seawater rather than freshwater.
Accepted: 7 March 2000 |
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Keywords: | Calcium Capillary electrophoresis Hemolymph volume Isopod Molting |
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