Mobilization of CD34~+CD38~- hematopoietic stem cells after priming in acute myeloid leukemia |
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Authors: | Adriana Plesa Youcef Chelghoum Eve Mattei Hélène Labussière Mohamed Elhamri Giovanna Cannas Stéphane Morisset Inès Tagoug Mauricette Michallet Charles Dumontet Xavier Thomas |
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Institution: | Adriana Plesa;Eve Mattei;Charles Dumontet;Youcef Chelghoum;Hélène Labussière;Giovanna Cannas;Mauricette Michallet;Xavier Thomas;Mohamed Elhamri;Stéphane Morisset;Inès Tagoug;Laboratoire de Cytologie et d’Immunologie, Centre Hospitalier Lyon-Sud, Hospices Civils de Lyon;Department of Hematology, Centre Hospitalier Lyon-Sud,Hospices Civils de Lyon;Unité de Recherche Clinique, Service d’Hématologie, Centre Hospitalier Lyon-Sud,Hospices Civils de Lyon;INSERM U590,Laboratoire de Cytologie Analy-tique, Faculté de Médecine Rockefeller, Université Lyon-I; |
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Abstract: | AIM: To evaluate quantitatively and qualitatively the different CD34+ cell subsets after priming by chemotherapy granulocyte colony-stimulating factor (± G-CSF) in patients with acute myeloid leukemia.METHODS: Peripheral blood and bone marrow samples were harvested in 8 acute myeloid leukemia patients during and after induction chemotherapy. The CD34/CD38 cell profile was analyzed by multi-parameter flow cytometry. Adhesion profile was made using CXC chemokine receptor 4 (CXCR4) (CD184), VLA-4 (CD49d/CD29) and CD47.RESULTS: Chemotherapy ± G-CSF mobilized immature cells (CD34+CD38− population), while the more mature cells (CD34+CD38low and CD34+CD38+ populations) decreased progressively after treatment. Circulating CD34+ cells tended to be more sensitive to chemotherapy after priming with G-CSF. CD34+ cell mobilization was correlated with a gradual increase in CXCR4 and CD47 expression, suggesting a role in cell protection and the capacity of homing back to the marrow.CONCLUSION: Chemotherapy ± G-CSF mobilizes into the circulation CD34+ bone marrow cells, of which, the immature CD34+CD38– cell population. Further manipulations of these interactions may be a means with which to control the trafficking of leukemia stem cells to improve patients’ outcomes. |
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Keywords: | Acute myeloid leukemia Leukemia stem cell Immunophenotype Priming Timed sequential chemotherapy |
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