Human hair follicle-derived mesenchymal stem cells: Isolation,expansion, and differentiation |
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Authors: | Bo Wang Xiao-Mei Liu Zi-Nan Liu Yuan Wang Xing Han Ao-Bo Lian Ying Mu Ming-Hua Jin Jin-Yu Liu |
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Institution: | Bo Wang, Xiao-Mei Liu, Zi-Nan Liu, Yuan Wang, Xing Han, Ao-Bo Lian, Ming-Hua Jin, Jin-Yu Liu, Department of Toxicology, School of Public Health, Jilin University, Changchun 130021, Jilin Province, ChinaYing Mu, Research Center for Analytical Instrumentation, Institute of Cyber-Systems and Control, State Key Laboratory of Industrial Control Technology, Zhejiang University, Hangzhou 310000, Zhejiang Province, China |
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Abstract: | Hair follicles are easily accessible skin appendages that protect against cold and potential injuries. Hair follicles contain various pools of stem cells, such as epithelial, melanocyte, and mesenchymal stem cells (MSCs) that continuously self-renew, differentiate, regulate hair growth, and maintain skin homeostasis. Recently, MSCs derived from the dermal papilla or dermal sheath of the human hair follicle have received attention because of their accessibility and broad differentiation potential. In this review, we describe the applications of human hair follicle-derived MSCs (hHF-MSCs) in tissue engineering and regenerative medicine. We have described protocols for isolating hHF-MSCs from human hair follicles and their culture condition in detail. We also summarize strategies for maintaining hHF-MSCs in a highly proliferative but undifferentiated state after repeated in vitro passages, including supplementation of growth factors, 3D suspension culture technology, and 3D aggregates of MSCs. In addition, we report the potential of hHF-MSCs in obtaining induced smooth muscle cells and tissue-engineered blood vessels, regenerated hair follicles, induced red blood cells, and induced pluripotent stem cells. In summary, the abundance, convenient accessibility, and broad differentiation potential make hHF-MSCs an ideal seed cell source of regenerative medical and cell therapy. |
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Keywords: | Human hair follicle Regenerative therapy Mesenchymal stem cell Tissue engineering Cell differentiation |
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| 点击此处可从《World journal of stem cells》浏览原始摘要信息 |
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