Serological characterization and partial purification of an Lyt-1 homolog in tunicate hemocytes. |
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Authors: | H I Negm M H Mansour E L Cooper |
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Institution: | Department of Anatomy and Cell Biology, School of Medicine, University of California, Los Angeles 90024-1763. |
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Abstract: | A panel of alloantisera and monoclonal antibodies specific to murine Lyt-1 allotypic and framework determinants was used in indirect immunofluorescence and FACS analysis to investigate the occurrence of an Lyt-1 homolog in tunicate (protochordate) hemocytes. Binding assays and quantitative absorption experiments established the expression of Lyt-1 cross-reacting determinants on a distinct population of tunicate hemocytes. These determinants were expressed exclusively by cells with the morphological characteristics of hemoblasts and lymphocytes. In a rapid two-step purification procedure, Lyt-1 glycoproteins from tunicate hemocytes and C57B1/6 mouse thymocytes were solubilized and partially purified by affinity chromatography using a mAb anti-Lyt-1 frame-work determinant. In both cell types, antigenic activities were associated with a major 67-kDa component. Our findings suggest an early phylogenetic emergence of an Lyt-1 homolog at this level of evolution. |
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Keywords: | Lyt-1 hemocytes purification evolution lymphocytes |
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